2. Academic Validation
  3. Bifendate inhibits autophagy at multiple steps and attenuates oleic acid-induced lipid accumulation

Bifendate inhibits autophagy at multiple steps and attenuates oleic acid-induced lipid accumulation

  • Biochem Biophys Res Commun. 2022 Nov 26:631:115-123. doi: 10.1016/j.bbrc.2022.09.067.
Weigang Yuan 1,  Fenglei Jian 2,  Yueguang Rong 3
Affiliations

Affiliations

  • 1 Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
  • 2 Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. Electronic address: fengleijian1990@163.com.
  • 3 Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. Electronic address: rongyueguang@hust.edu.cn.
PMID: 36183552 DOI: 10.1016/j.bbrc.2022.09.067
Abstract

Some traditional Chinese medicines exert roles in the therapy of liver diseases by modulating Autophagy. Bifendate (DDB), a synthetic intermediate of Schisandrin C extracted from Schisandrae chinensis, is clinically used to treat hepatitis in China. While DDB is a positive control to research some potential hepatoprotective agents, its related molecular mechanisms are unknown. In this study, we show that DDB inhibited autophagosome-lysosome fusion, lysosome acidification and autophagic lysosome reformation. Moreover, DDB attenuated oleic acid-induced lipid droplet accumulation. These findings reveal the effects of DDB on the autophagy-related processes and lysosomal function, and also provide a possibility to understand the bioactivity mechanism of DDB in the future.

Keywords

Autophagy; Bifendate; Lipid droplet; Lysosome.

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