2. Academic Validation
  3. Fluxapyroxad disrupt erythropoiesis in zebrafish (Danio rerio) embryos

Fluxapyroxad disrupt erythropoiesis in zebrafish (Danio rerio) embryos

  • Ecotoxicol Environ Saf. 2022 Dec 1:247:114259. doi: 10.1016/j.ecoenv.2022.114259.
Xin Chen 1,  Tiantong Qiu 1,  Mengjun Pan 1,  Peng Xiao 2,  Wenhua Li 3
Affiliations

Affiliations

  • 1 Engineering Research Center of Molecular Medicine of Ministry of Education, Key Laboratory of Fujian Molecular Medicine, Key Laboratory of Xiamen Marine and Gene Drugs, Key Laboratory of Precision Medicine and Molecular Diagnosis of Fujian Universities, School of Biomedical Sciences, Huaqiao University, Xiamen 361021, PR China.
  • 2 National and Local Joint Engineering Research Center of Ecological Treatment Technology for Urban Water Pollution, Zhejiang Provincial Key Lab for Water Environment and Marine Biological Resources Protection, College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, PR China. Electronic address: pxiao@wzu.edu.cn.
  • 3 Engineering Research Center of Molecular Medicine of Ministry of Education, Key Laboratory of Fujian Molecular Medicine, Key Laboratory of Xiamen Marine and Gene Drugs, Key Laboratory of Precision Medicine and Molecular Diagnosis of Fujian Universities, School of Biomedical Sciences, Huaqiao University, Xiamen 361021, PR China. Electronic address: wenhuali@hqu.edu.cn.
PMID: 36334343 DOI: 10.1016/j.ecoenv.2022.114259
Abstract

Fluxapyroxad, a Succinate Dehydrogenase Inhibitor (SDHI) fungicide, is commercialized worldwide to control a variety of Fungal diseases. Growing evidence shows that fluxapyroxad is teratogenic to aquatic organisms. In this study, the influence of fluxapyroxad toward hematopoietic development was evaluated using zebrafish embryos which were exposed to fluxapyroxad (0.03 µM, 0.3 µM and 3 µM) from 3 h post fertilization (hpf) to 3 days post fertilization (dpf). Compared to the control groups, the hemoglobin was ectopic and decreased in response to fluxapyroxad treatment. The transcription levels of genes (hbbe1, hbbe2, and gata1a) involved in erythropoiesis were reduced after exposure to fluxapyroxad. In contrast, the distributions and expression of marker genes for myeloid lineage cells were unaffected by fluxapyroxad exposure. Our data suggested that fluxapyroxad might specifically affect erythropoiesis and hold great promise for the assessment of the toxicity of fluxapyroxad to aquatic organisms.

Keywords

Embryo; Fluxapyroxad; Hematotoxicity; SDHIs; Zebrafish.

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