2. Academic Validation
  3. An inhibitory effect of schisandrone on α-hemolysin expression to combat methicillin-resistant staphylococcus aureus infections

An inhibitory effect of schisandrone on α-hemolysin expression to combat methicillin-resistant staphylococcus aureus infections

  • World J Microbiol Biotechnol. 2022 Nov 8;39(1):3. doi: 10.1007/s11274-022-03442-x.
Yingxin Qi 1 2,  Juan Hou 3,  Yicheng Zhao 3 4,  Wu Song 3,  Li Wang 3,  Huan Chen 5,  Guang Chen 6 7
Affiliations

Affiliations

  • 1 College of Life Science, Key Laboratory of Straw Biology and Utilization of the Ministry of Education Jilin Agricultural University, Changchun, China.
  • 2 School of Biological and Food Engineering, Jilin Engineering Normal University, Changchun, China.
  • 3 College of Clinical Medicine, Changchun University of Chinese Medicine, Changchun, China.
  • 4 Center for Pathogen Biology and Infectious Diseases, Key Laboratory of Organ Regeneration and Transplantation of the Ministry of Education, The First Hospital of Jilin University, Changchun, China.
  • 5 Jilin Agricultural University, Changchun, China.
  • 6 College of Life Science, Key Laboratory of Straw Biology and Utilization of the Ministry of Education Jilin Agricultural University, Changchun, China. chenguang_jlau@163.com.
  • 7 School of Biological and Food Engineering, Jilin Engineering Normal University, Changchun, China. chenguang_jlau@163.com.
PMID: 36344903 DOI: 10.1007/s11274-022-03442-x
Abstract

Due to increasing Antibiotic resistance, targeting Bacterial virulence factors is now gaining further interest as an alternative strategy to develop novel classes of anti-infective agents. The critical role of α-hemolysin (Hla), an indispensable virulence determinant, in the pathogenicity of Staphylococcus aureus renders this virulence factor an appealing target for effective therapeutic applications. Herein, we identified a natural compound schisandraone, as an effective Hla inhibitor, which could inhibit Hla production and thus hemolytic activity in a dose-dependent manner without affecting the growth of S. aureus. We also found that the addition of schisandrone could down-regulate the transcriptional levels of the hla, agrA and RNAIII and significantly alleviated Hla-mediated injury of A549 cells co-cultured with S. aureus. In vivo studies further suggested that schisandrone combined with Antibiotic ceftiofur exhibited a significant therapeutic effect on S. aureus Infection. These findings revealed the role of schisandrone in inhibiting the activity of Hla and we believe that it is a promising anti-virulence candidate to combat MRSA pneumonia.

Keywords

Anti-infection; Anti-virulence; Mice pneumonia model; α-Hemolysin.

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