2. Academic Validation
  3. Consumption of Saturated Fatty Acids-Rich Lard Benefits Recovery of Experimental Arthritis by Activating PPAR-γ

Consumption of Saturated Fatty Acids-Rich Lard Benefits Recovery of Experimental Arthritis by Activating PPAR-γ

  • Mol Nutr Food Res. 2022 Nov 25;e2200429. doi: 10.1002/mnfr.202200429.
Rui Wang 1,  Cong-Lan Ji 2,  Dan-Dan Feng 3,  Yi-Jin Wu 4,  Yan Li 1,  Opeyemi Joshua Olatunji 5,  Li-Jun Yu 4,  Jian Zuo 1 6 7
Affiliations

Affiliations

  • 1 Xin'an Medicine Research Center, the First Affiliated Hospital of Wannan Medical College (Yijishan Hospital), Wuhu, 241000, China.
  • 2 School of Pharmacy, Anhui College of Traditional Chinese Medicine, Wuhu, Anhui, 241000, China.
  • 3 Department of Rheumatology, the First Affiliated Hospital of Wannan Medical College (Yijishan Hospital), Wuhu, 241000, China.
  • 4 Research Center of Integration of Traditional Chinese and Western Medicine, Wannan Medical College, Wuhu, 241000, China.
  • 5 Traditional Thai Medical Research and Innovation Center, Faculty of Traditional Thai Medicine, Prince of Songkla University, Hat Yai, 90110, Thailand.
  • 6 Key Laboratory of Non-coding RNA Transformation Research of Anhui Higher Education Institution, Wannan Medical College, Wuhu, 241000, China.
  • 7 Anhui Provincial Engineering Laboratory for Screening and Re-evaluation of Active Compounds of Herbal Medicines in Southern Anhui, Wuhu, 241000, China.
PMID: 36433679 DOI: 10.1002/mnfr.202200429
Abstract

Scope: This study investigated the impacts of lard and related fatty acids intake on rheumatoid arthritis (RA) animal models.

Method and results: Collagen-induced arthritis (CIA) and adjuvant-induced arthritis (AIA) were induced in SD rats and C57 BL/6 mice respectively, which were fed by lard-rich diet (LRD) for 42 days with intake restriction or not. AIA SD rats were treated by representative fatty acids for 30 days. Body weight, arthritis score and metabolic profile were periodically recorded. Monocyte distribution, cytokine/metabolites levels, gene expression and tissue damages were investigated by flow cytometry, ELISA, colorimetry, PCR and histological methods. After being treated by fatty acids in vitro, THP-1 monocytes and the corresponding medium were collected for ELISA, PCR, immunoblotting and reporter gene assays. Irrespective of intake amounts, LRD decreased inflammatory cytokines and inhibited glycolysis in all rheumatic rodents. Furthermore, it altered monocyte distribution and promoted PPAR-γ expression in AIA mice. Overall evidences show that both saturated (SF) and unsaturated fatty acids (USF) from lard can attenuate inflammation by activating PPAR-γ. Silencing PPAR-γ abrogated their anti-inflammatory effects in vitro. Besides, SF can stimulate TLR4/NF-κB pathway.

Conclusion: Lard consumption is beneficial for active inflammatory arthritis recovery. Even SF can activate PPAR-γ and consequently attenuate inflammation. This article is protected by copyright. All rights reserved.

Keywords

PPAR-γ; TLR4; energy metabolism; fatty acids; inflammation; monocytes; rheumatoid arthritis.

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