1. Academic Validation
  2. Antidepressant-like effects of geniposide in chronic unpredictable mild stress-induced mice by regulating the circ_0008405/miR-25-3p/Gata2 and Oip5os1/miR-25-3p/Gata2 networks

Antidepressant-like effects of geniposide in chronic unpredictable mild stress-induced mice by regulating the circ_0008405/miR-25-3p/Gata2 and Oip5os1/miR-25-3p/Gata2 networks

  • Phytother Res. 2022 Dec 14. doi: 10.1002/ptr.7702.
Yu Zhao 1 Qian Zhang 2 Yuzhu Yan 1 Xinbo Wang 1 Yin Shao 1 Cheng Mei 3 Tianyu Zou 4
Affiliations

Affiliations

  • 1 Department of Acupuncture, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, PR China.
  • 2 Department of Acupuncture, Second Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, PR China.
  • 3 Department of Encephalopathy, Heilongjiang Academy of Chinese Medical Sciences, Harbin, PR China.
  • 4 Department of Encephalopathy, Shenzhen Hospital of Shanghai University of Traditional Chinese Medicine, Shenzhen, PR China.
Abstract

Evidence exists suggesting the anti-depressive activities of geniposide (GP), a major compound in Gardenia jasminoides Ellis. Accordingly, the present study attempts to explore the anti-depressive mechanism of GP in chronic unpredictable mild stress (CUMS)-induced depression-like behaviors of mice. CUMS-induced mice were given GP daily and subjected to behavioral tests to observe the effect of GP on the depression-like behaviors. It was noted that GP administration reduced depression-like behaviors in CUMS mice. Transcriptome Sequencing was conducted in three control and three CUMS mice. Differentially expressed circRNAs, lncRNAs and mRNAs were then screened by bioinformatics analyses. Intersection analysis of the transcriptome Sequencing results with the bioinformatics analysis results was followed to identify the candidate targets. We found that Gata2 alleviated depression-like behaviors via the metabolism- and synapse-related pathways. Gata2 was a target of miR-25-3p, which had binding sites to circ_0008405 and Oip5os1. circ_0008405 and Oip5os1 competitively bound to miR-25-3p to release the expression of Gata2. GP administration ameliorated depression-like behaviors in CUMS mice through regulation of the circ_0008405/miR-25-3p/Gata2 and Oip5os1/miR-25-3p/Gata2 crosstalk networks. Taken together, GP may exert a potential antidepressant-like effect on CUMS mice, which is ascribed to regulation of the circ_0008405/miR-25-3p/Gata2 and Oip5os1/miR-25-3p/Gata2 crosstalk networks.

Keywords

Gata2; Oip5os1; chronic unpredictable mild stress; circ_0008405; depression-like behavior; miR-25-3p; transcriptome sequencing.

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