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  3. Generation of Human Trophoblast Stem Cell-Dependent Placental In Vitro Models

Generation of Human Trophoblast Stem Cell-Dependent Placental In Vitro Models

  • Methods Mol Biol. 2022 Dec 15. doi: 10.1007/7651_2022_463.
Hao Wu 1 2 3 4,  Yue Wang 1 2 3 4,  Hongmei Wang 5 6 7 8
Affiliations

Affiliations

  • 1 State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
  • 2 Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China.
  • 3 University of Chinese Academy of Sciences, Beijing, China.
  • 4 Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, China.
  • 5 State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. wanghm@ioz.ac.cn.
  • 6 Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China. wanghm@ioz.ac.cn.
  • 7 University of Chinese Academy of Sciences, Beijing, China. wanghm@ioz.ac.cn.
  • 8 Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, China. wanghm@ioz.ac.cn.
PMID: 36515896 DOI: 10.1007/7651_2022_463
Abstract

Currently, human trophoblast stem cell (hTSC) is considered to be the most promising laboratory model stimulating trophoblast criteria. Our group has established hTSCs allowing differentiation to syncytiotrophoblast (STB) and extravillous trophoblast (EVT). Further, hTSC-based three-dimensional (3D) trophoblast Organoid (hTSC-organoid) provides a transformative model for studying human placental development and the interaction between trophoblast and maternal environment. Here, we present a protocol to obtain different types of placental trophoblast cells and trophoblast organoids using hTSCs. The generation of hTSC-organoids takes 6 days. hTSC-organoids permit passaging and can differentiate into EVT lineage.

Keywords

Human trophoblast organoid; Human trophoblast stem cell.

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