2. Academic Validation
  3. Bifidobacterium longum CCFM1206 Promotes the Biotransformation of Glucoraphanin to Sulforaphane That Contributes to Amelioration of Dextran-Sulfate-Sodium-Induced Colitis in Mice

Bifidobacterium longum CCFM1206 Promotes the Biotransformation of Glucoraphanin to Sulforaphane That Contributes to Amelioration of Dextran-Sulfate-Sodium-Induced Colitis in Mice

  • J Agric Food Chem. 2023 Jan 5. doi: 10.1021/acs.jafc.2c07090.
Jiaying Wu 1 2,  Shumao Cui 1 2,  Xin Tang 1 2,  Qiuxiang Zhang 1 2,  Yan Jin 3,  Jianxin Zhao 1 2,  Bingyong Mao 1 2,  Hao Zhang 1 2 4
Affiliations

Affiliations

  • 1 State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
  • 2 School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
  • 3 The Affiliated Wuxi Second People's Hospital of Nanjing Medical University, Wuxi, Jiangsu 214002, People's Republic of China.
  • 4 National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.
PMID: 36604158 DOI: 10.1021/acs.jafc.2c07090
Abstract

Glucoraphanin, rich in broccoli seed extract (BSE), is generally inert but can be biotransformed into active sulforaphane by gut bacteria. This study aimed to screen probiotics with glucoraphanin-metabolizing ability and explore the effect of a combination of strain and BSE on colitis induced by dextran sulfate sodium (DSS) in mice. Bifidobacterium longum CCFM1206 was isolated from healthy adult feces. Ultra-high-performance liquid chromatography Q Exactive mass spectrometry analysis revealed the presence of sulforaphane, sulforaphane-l-cysteine, and erucin in the BSE supernatant fermented by B. longum CCFM1206 in vitro. Combined and individual interventions of BSE and B. longum CCFM1206 were applied to explore the effects on DSS-induced colitis. The results suggested that the combination of B. longum CCFM1206 and BSE could ameliorate colitis symptoms, relieve colonic inflammatory reactions and oxidative stress, and protect the intestinal barrier in DSS-induced mice. In comparison to the BSE intervention alone, the combined intervention of B. longum CCFM1206 and BSE promoted the generation of sulforaphane and sulforaphane-N-acetylcysteine in mice colon from 220.88 ± 19.81 to 333.99 ± 36.46 nmol/g and from 232.04 ± 26.48 to 297.50 ± 40.08 nmol/g dry weight feces, respectively. According to quantitative reverse transcription polymerase chain reaction and immunohistochemical analysis, B. longum CCFM1206 and BSE effectively activated the transcription and expression of genes related to the Nrf2 signaling pathway. These results were intended to elucidate that probiotics could elevate the bioactivity of dietary phytochemicals in vivo, and the combination had potential for therapeutic treatment of colitis.

Keywords

Bifidobacterium longum; broccoli seed extract; glucoraphanin; probiotics; sulforaphane; ulcerative colitis.

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