2. Academic Validation
  3. PbO nanoparticles increase the expression of ICAM-1 and VCAM-1 by increasing reactive oxygen species production in choroid plexus

PbO nanoparticles increase the expression of ICAM-1 and VCAM-1 by increasing reactive oxygen species production in choroid plexus

  • Environ Sci Pollut Res Int. 2023 Jan 6. doi: 10.1007/s11356-022-25109-8.
Weixuan Wang 1,  Shuang Li 1 2,  Xi Wang 1,  Jianbo Wang 1,  Yanshu Zhang 3 4
Affiliations

Affiliations

  • 1 School of Public Health, North China University of Science and Technology, Tangshan, China.
  • 2 The Experiment Animal Center, North China University of Science and Technology, Tangshan, China.
  • 3 School of Public Health, North China University of Science and Technology, Tangshan, China. yanshuzhang@ncst.edu.cn.
  • 4 The Experiment Animal Center, North China University of Science and Technology, Tangshan, China. yanshuzhang@ncst.edu.cn.
PMID: 36607576 DOI: 10.1007/s11356-022-25109-8
Abstract

PbO nanoparticles (nano-PbO) are widely used in the production of electrode Materials, but exposure to them can cause brain damage. The first barrier preventing nano-PbO from entering the brain is the choroid plexus. However, the effect of nano-PbO on the choroid plexus remains unclear. Thus, the purpose of this study was to investigate the effect of nano-PbO exposure on lymphocyte cells infiltration, the adhesion protein of the choroid plexus as well as the role of Reactive Oxygen Species (ROS) during the process. Results showed that nano-PbO exposure increased the percentage of lymphocyte cells in the brain and upregulated the expression of surface adhesion proteins, including intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in choroid plexus. Meanwhile, nano-PbO treatment also resulted in the increase of intercellular ROS production, and significantly decrease glutathione (GSH) content, Glutathione Peroxidase (GSH-PX) activity, and superoxide dismutase (SOD) activity in Z310 cells beside the increase of ICAM and VCAM-1 expression. Treatment with ROS inhibitor N-acetylcysteine (NAC) significantly downregulated the expression of ICAM-1 and VCAM-1expression. In conclusion, exposure to nano-PbO increases the expression of ICAM-1 and VCAM-1 through oxidative stress, which may contribute to peripheral lymphocyte cells infiltration into the brain.

Keywords

Choroid plexus; ICAM-1; NAC; Nano-PbO; ROS; VCAM-1.

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