2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease Apoptosis Anti-infection
  3. Reactive Oxygen Species Endogenous Metabolite Apoptosis Ferroptosis Influenza Virus
  4. Acetylcysteine

Acetylcysteine  (Synonyms: N-Acetylcysteine; N-Acetyl-L-cysteine; NAC)

Cat. No.: HY-B0215 Purity: 99.44%
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Acétylcystéine (N-acétyl-L-cystéine) est un agent mucolytique qui réduit l'épaisseur du mucus. Acétylcystéine est un inhibiteur des ROS. Acétylcystéine est un précurseur de la cystéine, prévient la ferroptose induite par l'hémine en neutralisant les lipides toxiques générés par l'activité dépendante de l'arachidonate des 5-lipoxygénases. Acétylcystéine induit l'apoptose cellulaire.

Acetylcystein ist ein mucolytic agent, das die Dicke des Schleims reduziert. Acetylcystein ist ein ROS-Inhibitor. Acetylcystein ist ein Cysteinvorläufer, der die durch Hämin induzierte ferroptosis verhindert, indem er toxische Lipide neutralisiert, die durch die Arachidonat-abhängige Aktivität von 5-Lipoxygenasen erzeugt werden. Acetylcystein induziert die apoptosis.

Acetylcysteine (N-Acetylcysteine) is a mucolytic agent which reduces the thickness of the mucus. Acetylcysteine is a ROS inhibitor. Acetylcysteine is a cysteine precursor, prevents hemin-induced ferroptosis by neutralizing toxic lipids generated by arachidonate-dependent activity of 5-lipoxygenases. Acetylcysteine induces cell apoptosis. Acetylcysteine also has anti-influenza virus activities.

For research use only. We do not sell to patients.

Acetylcysteine Chemical Structure

Acetylcysteine Chemical Structure

CAS No. : 616-91-1

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 USD 33 In-stock
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10 mM * 1 mL in Water USD 33 In-stock
Solid
500 mg USD 30 In-stock
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10 g USD 50 In-stock
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Based on 392 publication(s) in Google Scholar

Other Forms of Acetylcysteine:

Acetylcysteine Related Antibodies

Top Publications Citing Use of Products

389 Publications Citing Use of MCE Acetylcysteine

WB

    Acetylcysteine purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2018 Apr 27;4:47.  [Abstract]

    Wild-type, Prx1KO, and Prx1OE cells are each divided into three groups, which are control groups, groups treated with 100 ng/mL LPS, and groups treated with 100 ng/mL LPS and NAC. Western blot analysis is then performed with antibodies against Bax, Bcl-2, Cyto-c, caspase 3, and GAPDH, and band intensity is measured with densitometry.

    Acetylcysteine purchased from MedChemExpress. Usage Cited in: Fundam Clin Pharmacol. 2017 Feb;31(1):64-74.  [Abstract]

    Advanced oxidation production product (AOPP)-triggered apoptosis-related signals are triggered by ROS production. Human chondrocytes are pretreated with N-Acetylcysteine (NAC; 5 mM) for 60 min, and then, chondrocyte are co-incubated with AOPP and NAC for 24 h. (a) Pretreatment of ROS scavenger NAC markedly alleviates AOPP-triggered mitochondria-mediated apoptosis proteins. (b) Pretreatment of chondrocyte with NAC abrogates the intracellular Ca2+ mobilization. (c) NAC pretreatment atte

    Acetylcysteine purchased from MedChemExpress. Usage Cited in: Sci Rep. 2016 Oct 27;6:35995.  [Abstract]

    Pretreatment of NAC, a free radical scavenger, largely decreases the expression of caspase-3 and almost abolishes the H2O2 induced upregulation of PRX1. Western blot analysis shows the pretreatment of osteoblast cells with E2 partially abrogates the increased PRX1 and caspase-3 expressions induced by H2O2.

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    Description

    Acetylcysteine (N-Acetylcysteine) is a mucolytic agent which reduces the thickness of the mucus. Acetylcysteine is a ROS inhibitor[1]. Acetylcysteine is a cysteine precursor, prevents hemin-induced ferroptosis by neutralizing toxic lipids generated by arachidonate-dependent activity of 5-lipoxygenases[5]. Acetylcysteine induces cell apoptosis[2][3]. Acetylcysteine also has anti-influenza virus activities[7].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    Acetylcysteine prevents apoptotic DNA fragmentation and maintains long-term survival in the absence of other trophic support in serum-deprived PC12 cells. Acetylcysteine also prevents death of PC12 cells and sympathetic neurons[2].
    Acetylcysteine causes dose-dependent reductions in viability in rat and human aortic smooth muscle cells[3].
    Acetylcysteine activates the Ras-extracellular signal-regulated kinase (ERK) pathway in PC12 cells. Acetylcysteine protects neuronal cells from death evoked by withdrawal of trophic support. Acetylcysteine increases nitric oxide (NO) release from protein-bound stores in vascular tissue. Acetylcysteine pretreatment of PC12 cells interferes with NGF-dependent signaling and neurite outgrowth, and it is suggested that Acetylcysteine interferes with redox-sensitive steps in the NGF mechanism[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Acetylcysteine Related Antibodies
    In Vivo

    Acetylcysteine (150, 300 mg/kg) treatment significantly reduces liver transaminases in all groups of treatment, mostly in group Acetylcysteine 300 mg/kg. Lung glutathione peroxidase is significantly increases in group Acetylcysteine 300 mg/kg(P=0.04), while the other oxidation biomarkers show no significant differences[6].
    Acetylcysteine improves cognition of 12-month-old SAMP8 mice in both the T-maze footshock avoidance paradigm and the lever press appetitive task without inducing non-specific effects on motor activity, motivation to avoid shock, or body weight[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Molecular Weight

    163.19

    Formula

    C5H9NO3S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    SC[C@@H](C(O)=O)NC(C)=O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (612.76 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 6.1276 mL 30.6382 mL 61.2764 mL
    5 mM 1.2255 mL 6.1276 mL 12.2553 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    Concentration (final)

    C2

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    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 120 mg/mL (735.32 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.44%

    COA (249 KB) HNMR (123 KB) CNMR (134 KB) RP-HPLC (277 KB) MS (69 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [2]

    For survival experiments, washed cells are resuspended in RPM1 1640 medium and plated in 0.5 mL at a density of 8-10×105 per well in 24 well plastic culture dishes coated with rat tail collagen. To feed, but to avoid loss of floating cells, fresh medium (0.2 mL) is added to the cultures on days 1, 5, and 10. For experiments involving "primed" PC12 cells, cultures are pretreated for l-2 weeks with NGF in RPM1 1640 medium supplemented with 1% heat-iN-acetylcysteinetivated horse serum. The cells are then washed and passaged into serum-free RPM1 1640 medium.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [6]

    Rats: Rats are randomLy allocated into five groups: sham group (n=5), control group with IIR (n=8) and three groups with IIR who are given Acetylcysteine in different dosages: 150 mg/kg intraperitoneally 5 min before ischemia (n=8, group Acetylcysteine 150), 300 mg/kg i.p 5 min before ischemia (n=7, group Acetylcysteine 300), and 150 mg/kg i.p 5 min before ischemia plus 150 mg/kg 5 min before reperfusion (n=7, group Acetylcysteine 150 + 150). After 4 h of reperfusion, the animals are euthanized by exsanguination from the abdominal aorta.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 6.1276 mL 30.6382 mL 61.2764 mL 153.1911 mL
    5 mM 1.2255 mL 6.1276 mL 12.2553 mL 30.6382 mL
    10 mM 0.6128 mL 3.0638 mL 6.1276 mL 15.3191 mL
    15 mM 0.4085 mL 2.0425 mL 4.0851 mL 10.2127 mL
    20 mM 0.3064 mL 1.5319 mL 3.0638 mL 7.6596 mL
    25 mM 0.2451 mL 1.2255 mL 2.4511 mL 6.1276 mL
    30 mM 0.2043 mL 1.0213 mL 2.0425 mL 5.1064 mL
    40 mM 0.1532 mL 0.7660 mL 1.5319 mL 3.8298 mL
    50 mM 0.1226 mL 0.6128 mL 1.2255 mL 3.0638 mL
    60 mM 0.1021 mL 0.5106 mL 1.0213 mL 2.5532 mL
    80 mM 0.0766 mL 0.3830 mL 0.7660 mL 1.9149 mL
    100 mM 0.0613 mL 0.3064 mL 0.6128 mL 1.5319 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Acetylcysteine Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Acetylcysteine616-91-1N-Acetylcysteine N-Acetyl-L-cysteine NACReactive Oxygen SpeciesEndogenous MetaboliteApoptosisFerroptosisInfluenza VirusInhibitorinhibitorinhibit

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