2. Academic Validation
  3. Protective Effects of Auraptene against Free Radical-Induced Erythrocytes Damage

Protective Effects of Auraptene against Free Radical-Induced Erythrocytes Damage

  • J Pharmacopuncture. 2022 Dec 31;25(4):344-353. doi: 10.3831/KPI.2022.25.4.344.
Khadijeh Jamialahmadi 1 2,  Amir Hossein Amiri 3,  Fatemeh Zahedipour 2 4,  Fahimeh Faraji 5,  Gholamreza Karimi 6 7
Affiliations

Affiliations

  • 1 Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
  • 2 Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
  • 3 Cancer Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
  • 4 Student Research Committee, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
  • 5 School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
  • 6 Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
  • 7 Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
PMID: 36628343 DOI: 10.3831/KPI.2022.25.4.344
Abstract

Objectives: Auraptene is the most abundant natural prenyloxycoumarin. Recent studies have shown that it has multiple biological and therapeutic properties, including antioxidant properties. Erythrocytes are constantly subjected to oxidative damage that can affect proteins and lipids within the erythrocyte membrane and lead to some hemoglobinopathies. Due to the lack of sufficient information about the antioxidant effects of auraptene on erythrocytes, this study intended to evaluate the potential of this compound in protecting radical-induced erythrocytes damages.

Methods: The antioxidant activity of auraptene was measured based on DPPH and FRAP assays. Notably, oxidative hemolysis of human erythrocytes was used as a model to study the ability of auraptene to protect biological membranes from free radical-induced damage. Also, the effects of auraptene in different concentrations (25-400 µM) on AAPH-induced lipid/protein peroxidation, glutathione (GSH) content and morphological changes of erythrocytes were determined.

Results: Oxidative hemolysis and lipid/protein peroxidation of erythrocytes were significantly suppressed by auraptene in a time and concentration-dependent manner. Auraptene prevented the depletion of the cytosolic antioxidant GSH in erythrocytes. Furthermore, it inhibited lipid and protein peroxidation in a time and concentration-dependent manner. Likewise, FESEM results demonstrated that auraptene reduced AAPH-induced morphological changes in erythrocytes.

Conclusion: Auraptene efficiently protects human erythrocytes against free radicals. Therefore, it can be a potent candidate for treating oxidative stress-related diseases.

Keywords

aaph; auraptene; hemolysis; oxidative stress; peroxidation.

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