1. Academic Validation
  2. Elevated PGT promotes proliferation and inhibits cell apoptosis in preeclampsia by Erk signaling pathway

Elevated PGT promotes proliferation and inhibits cell apoptosis in preeclampsia by Erk signaling pathway

  • Mol Cell Probes. 2023 Feb 2;67:101896. doi: 10.1016/j.mcp.2023.101896.
Huiyuan Pang 1 Di Lei 1 Jinfa Huang 1 Yuping Guo 2 Cuifang Fan 3
Affiliations

Affiliations

  • 1 Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei Province, PR China.
  • 2 Department of Obstetrics and Gynecology, First Affiliated Hospital of Gannan Medical University, Ganzhou, 341000, Jiangxi Province, PR China.
  • 3 Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei Province, PR China. Electronic address: fancuifang@whu.edu.cn.
Abstract

Prostaglandins participate in maternal recognition of pregnancy, implantation and maintenance of gestation. Prostaglandin transporter (PGT), as a candidate molecule of prostaglandin carriers, might be involved in the pathogenesis of preeclampsia. In preeclampsia (PE) patients' placental tissue, we identified PGT by RNA Sequencing, measured its expression pattern by quantitative Real-Time PCR and Western blot. PGT was found to be upregulated in preeclamptic placental tissue. The expression pattern of PGT in PE was double confirmed by eight Gene Expression Omnibus (GEO) databases. In abortion tissues at 6-8 weeks, we then observed the cellular location of PGT by Immunofluorescence technique (IF) and found PGT located in trophoblast cell of the placenta of early pregnancy. In vitro studies revealed that forced expression of PGT in HTR8/Sveno cell inhibited its Apoptosis, but promoted its proliferation by activating ERK signaling. In vivo study, we used reduced uterine perfusion pressure (RUPP) rat model and L-NAME-induced preeclampsia-like rats to study the possible role of PGT in preeclampsia. And PGT was found to be upregulated in both preeclampsia rat models by Immunohistochemical (IHC) staining. Newly identified PGT plays an important role in trophoblast proliferation via ERK signaling, providing new insights for understanding the pathogenesis of PE.

Keywords

Apoptosis; Erk pathway; PGT; Preeclampsia; Proliferation.

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