L-NAME hydrochloride (Synonyms: NG-Nitroarginine methyl ester hydrochloride)

Name: L-NAME hydrochloride
Brand: MedChemExpress
SKU: HY-18729A
Rating: 5.0 (48 reviews)

Cat. No.: HY-18729A Purity: 99.70%: FAQs
Data Sheet SDS COA Handling Instructions

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L-NAME hydrochloride inhibits NOS with an IC₅₀ of 70 μM. L-NAME is a precursor to NOS inhibitor L-NOARG which has an IC₅₀ value of 1.4 μM.

For research use only. We do not sell to patients.

L-NAME hydrochloride Chemical Structure

CAS No. : 51298-62-5

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	USD 61	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 61	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 35	In-stock	Estimated Time of Arrival: December 31
1 g	USD 50	In-stock	Estimated Time of Arrival: December 31
5 g	USD 140	In-stock	Estimated Time of Arrival: December 31
10 g	USD 200	In-stock	Estimated Time of Arrival: December 31
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Based on 48 publication(s) in Google Scholar

Other Forms of L-NAME hydrochloride:

L-NAME Get quote
L-NAME hydrochloride (Standard) Get quote

45 Publications Citing Use of MCE L-NAME hydrochloride

: L-NAME hydrochloride purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 Mar 13;161:114484. [Abstract]; L-NAME (10 mg/kg; i.p.; single daily for 7 days; pretreat) alleviates Candesartan-induced glomerular shrinkage and renal tubular necrosis and detachment, in low-salt rats.

: L-NAME hydrochloride purchased from MedChemExpress. Usage Cited in: Exp Ther Med. 2018 Aug;16(2):1079-1086. [Abstract]; Effect of L-name on the expression of eNOS, HSP90, Nrf2, Nqo1 and HO-1 in a rat model of spinal cord injury (SCI).

: L-NAME hydrochloride purchased from MedChemExpress. Usage Cited in: Bosn J Basic Med Sci. 2017 May 20;17(2):132-137. [Abstract]; Effects of Akt/adenosine monophosphate-activated protein kinaseon CGP 48933 (VAL)-mediated endothelial nitric oxide (NO) synthase (eNOS) phosphorylation and NO production in human umbilical vein endothelial cells (HUVECs). (A) The variation of VAL (10 μM)-induced eNOS activation after HUVECs are incubated with LY294002 [LY] (10 μM), Compound C (1 μM), L-NAME (500 μM) for 3 hours. (B)The variation of VAL (10 μM)-induced nitric oxide (NO) productionafter HUVECs are incubated with LY294002 (10 μM),

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nNOS iNOS eNOS

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References
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Description

L-NAME hydrochloride inhibits NOS with an IC₅₀ of 70 μM. L-NAME is a precursor to NOS inhibitor L-NOARG which has an IC₅₀ value of 1.4 μM.

IC₅₀ & Target

IC50: 70 μM (NOS)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
Sf9	IC₅₀	0.68 μM Compound: L-NAME	Inhibition of human recombinant eNOS expressed in Sf9 cells assessed as inhibition of conversion of [3H]-L-arginine to [3H]-L-citrulline after 45 mins by liquid scintillation counting Inhibition of human recombinant eNOS expressed in Sf9 cells assessed as inhibition of conversion of [3H]-L-arginine to [3H]-L-citrulline after 45 mins by liquid scintillation counting	[PMID: 21923116]
Sf9	IC₅₀	0.69 μM Compound: L-NAME	Inhibition of human recombinant nNOS expressed in Sf9 cells assessed as inhibition of conversion of [3H]-L-arginine to [3H]-L-citrulline after 45 mins by liquid scintillation counting Inhibition of human recombinant nNOS expressed in Sf9 cells assessed as inhibition of conversion of [3H]-L-arginine to [3H]-L-citrulline after 45 mins by liquid scintillation counting	[PMID: 21923116]
Sf9	IC₅₀	0.83 μM Compound: L-NAME	Inhibition of human recombinant iNOS expressed in Sf9 cells assessed as inhibition of conversion of [3H]-L-arginine to [3H]-L-citrulline after 45 mins by liquid scintillation counting Inhibition of human recombinant iNOS expressed in Sf9 cells assessed as inhibition of conversion of [3H]-L-arginine to [3H]-L-citrulline after 45 mins by liquid scintillation counting	[PMID: 21923116]

In Vitro

L-arginine analogues are widely used inhibitors of nitric oxide synthase (NOS) activity, with N^w-nitro-L-arginine methyl ester (L-NAME) being at the head^[2]. Freshly dissolved L-NAME is a 50 fold less potent inhibitor of purified brain NOS (mean IC₅₀= 70 μM) than L-NOARG (IC₅₀= 1.4 μM), but the apparent inhibitory potency of L-NAME approached that of L-NOARG upon prolonged incubation at neutral or alkaline pH. HPLC analyses reveal that NOS inhibition by L-NAME closely correlated with hydrolysis of the drug to L-NOARG^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

L-NAME hydrochloride can be used to induce hypertension models^[6].

Induction of hypertension Model^[6]

Background

L-NAME hydrochloride decreases nitric oxide (NO) release with an inhibition competence in endothelial nitric oxide synthase (eNOS) in animals.

Specific Mmodeling Methods

Mice: Swiss Webster • male • 6-week-old
Administration: 400 mg/kg • ip • once daily for 7 days

Note

Modeling Indicators

Body quality changes: Induced hypertension with body weight loss and high blood pressure.

Correlated Product(s): /

Opposite Product(s): /

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Sprague-Dawley (SD) rats ^[7]
Dosage:	40 mg/kg, 5 weeks
Administration:	drinking water
Result:	Induced hypertension with body weight loss and high blood pressure.

Clinical Trial

Molecular Weight

269.69

Formula

C₇H₁₆ClN₅O₄

CAS No.

51298-62-5

Appearance

Solid

Color

White to light yellow

SMILES

N[C@@H](CCCNC(N[N+]([O-])=O)=N)C(OC)=O.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (370.80 mM; Need ultrasonic)

DMSO : 100 mg/mL (370.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7080 mL	18.5398 mL	37.0796 mL
5 mM	0.7416 mL	3.7080 mL	7.4159 mL
10 mM	0.3708 mL	1.8540 mL	3.7080 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 140 mg/mL (519.11 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.70%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (245 KB) HNMR (252 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Pfeiffer S, et al. Inhibition of nitric oxide synthesis by NG-nitro-L-arginine methyl ester (L-NAME): requirement forbioactivation to the free acid, NG-nitro-L-arginine. Br J Pharmacol. 1996 Jul;118(6):1433-40. [Content Brief]

[2]. Kopincová J, et al. L-NAME in the cardiovascular system - nitric oxide synthase activator? Pharmacol Rep. 2012;64(3):511-20. [Content Brief]

[3]. Lo HC, et al. The Nitric Oxide Synthase Inhibitor NG-Nitro-L-Arginine Methyl Ester Diminishes the Immunomodulatory Effects of Parental Arginine in Rats with Subacute Peritonitis. PLoSOne. 2016 Mar 23;11(3):e0151973. [Content Brief]

[4]. Luo H, et al. Effect of nitric oxide synthase inhibitor L-NAME on fear extinction in rats: a task-dependent effect. Neurosci Lett. 2014 Jun 20;572:13-8. [Content Brief]

[5]. Ocsan RJ, et al. Chronic NG-nitro-l-arginine methyl ester (L-NAME) administration in C57BL/6J mice induces a sustained decrease in c-kit positive cells during development of cardiac hypertrophy. J Physiol Pharmacol. 2013 Dec;64(6):727-36. [Content Brief]

[6]. V A Peotta, et al. Cardiovascular neural reflexes in L-NAME-induced hypertension in mice. Hypertension. 2001, 38, 3. [Content Brief]

[7]. Xiaofei Li, et al. Fucoidan from Undaria pinnatifida prevents vascular dysfunction through PI3K/Akt/eNOS-dependent mechanisms in the l-NAME-induced hypertensive rat model. Food Funct. 2016, 7, 5. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.7080 mL	18.5398 mL	37.0796 mL	92.6990 mL
	5 mM	0.7416 mL	3.7080 mL	7.4159 mL	18.5398 mL
	10 mM	0.3708 mL	1.8540 mL	3.7080 mL	9.2699 mL
	15 mM	0.2472 mL	1.2360 mL	2.4720 mL	6.1799 mL
	20 mM	0.1854 mL	0.9270 mL	1.8540 mL	4.6350 mL
	25 mM	0.1483 mL	0.7416 mL	1.4832 mL	3.7080 mL
	30 mM	0.1236 mL	0.6180 mL	1.2360 mL	3.0900 mL
	40 mM	0.0927 mL	0.4635 mL	0.9270 mL	2.3175 mL
	50 mM	0.0742 mL	0.3708 mL	0.7416 mL	1.8540 mL
	60 mM	0.0618 mL	0.3090 mL	0.6180 mL	1.5450 mL
	80 mM	0.0463 mL	0.2317 mL	0.4635 mL	1.1587 mL
	100 mM	0.0371 mL	0.1854 mL	0.3708 mL	0.9270 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

L-NAME hydrochloride Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L-NAME51298-62-5NG-Nitroarginine methyl esterNO SynthaseNitric oxide synthasesNOSInhibitorinhibitorinhibit

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L-NAME hydrochloride (Synonyms: NG-Nitroarginine methyl ester hydrochloride)

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L-NAME hydrochloride Related Antibodies

45 Publications Citing Use of MCE L-NAME hydrochloride

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Purity & Documentation

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L-NAME hydrochloride Related Antibodies

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Complete Stock Solution Preparation Table

L-NAME hydrochloride Related Classifications

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