2. Academic Validation
  3. Bufadienolides from the Bufo viridis toad venom exert cytotoxic effects on cancer cells by inducing cell apoptosis and cell cycle arrest

Bufadienolides from the Bufo viridis toad venom exert cytotoxic effects on cancer cells by inducing cell apoptosis and cell cycle arrest

  • Toxicol In Vitro. 2023 Feb 3;89:105566. doi: 10.1016/j.tiv.2023.105566.
Qiang Dong 1,  Gulmira Turdu 1,  Naziermu Dongmulati 1,  Ayitila Maimaitijang 1,  Haji Akber Aisa 2,  Abulimiti Yili 3
Affiliations

Affiliations

  • 1 State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, and the Key Laboratory of Chemistry of Plant Resources in Arid Regions Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, South Beijing Road 40-1, Urumqi 830011, People's Republic of China; University of Chinese Academy of Sciences, No.19(A) Yuquan Road, Shijingshan District, Beijing, 100049, People's Republic of China.
  • 2 State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, and the Key Laboratory of Chemistry of Plant Resources in Arid Regions Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, South Beijing Road 40-1, Urumqi 830011, People's Republic of China.
  • 3 State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, and the Key Laboratory of Chemistry of Plant Resources in Arid Regions Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, South Beijing Road 40-1, Urumqi 830011, People's Republic of China. Electronic address: abu@ms.xjb.ac.cn.
PMID: 36738868 DOI: 10.1016/j.tiv.2023.105566
Abstract

A series of bufadienolides were isolated from the Bufo viridis toad venom, and their cytotoxic activities against three human Cancer cell lines (HeLa, HT-29, MCF7) and a non-cancer cell line (L-O2) were explored using the MTT assay in vitro. All of nine compounds exhibited cytotoxic activities against the three Cancer cell lines, with compound D4 exhibiting potent cytotoxic activity against HeLa cells and was better than positive control. Herein, we further evaluated the effect of compound D4 on HeLa cells. The results revealed that compound D4 has excellent cytotoxic effect on HeLa cells by inhibiting cell colony formation and migration, promoting cell Apoptosis, increasing Reactive Oxygen Species (ROS) levels and arresting of HeLa cells in S and G2/M phases. These findings encourage further work on the chemistry and bioactivity of the Bufo viridis toad venom.

Keywords

Arenobufagin; Bufadienolides; Bufo viridis toad venom; Cell cycle; Cytotoxic activity; ROS.

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