2. Academic Validation
  3. Desmethyl type germinone, a specific agonist for the HTL/KAI2 receptor, induces the Arabidopsis seed germination in a gibberellin-independent manner

Desmethyl type germinone, a specific agonist for the HTL/KAI2 receptor, induces the Arabidopsis seed germination in a gibberellin-independent manner

  • Biochem Biophys Res Commun. 2023 Mar 15:649:110-117. doi: 10.1016/j.bbrc.2023.01.086.
Shoma Okabe 1,  Kana Kitaoka 2,  Taiki Suzuki 1,  Michio Kuruma 3,  Shinya Hagihara 4,  Shinjiro Yamaguchi 5,  Kosuke Fukui 6,  Yoshiya Seto 7
Affiliations

Affiliations

  • 1 Laboratory of Plant Chemical Regulation, School of Agriculture, Meiji University, 1-1-1, Higashi-Mita, Tama-ku, Kawasaki, Kanagawa, Japan.
  • 2 Department of Biochemistry, Okayama University of Science, Okayama City, Okayama, 700-0005, Japan.
  • 3 Laboratory of Plant Chemical Regulation, School of Agriculture, Meiji University, 1-1-1, Higashi-Mita, Tama-ku, Kawasaki, Kanagawa, Japan; RIKEN Center for Sustainable Resource Science, 2-1 Hirosawa, Wako, Saitama, Japan.
  • 4 RIKEN Center for Sustainable Resource Science, 2-1 Hirosawa, Wako, Saitama, Japan.
  • 5 Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto, 611-0011, Japan.
  • 6 Department of Biochemistry, Okayama University of Science, Okayama City, Okayama, 700-0005, Japan. Electronic address: fukui@ous.ac.jp.
  • 7 Laboratory of Plant Chemical Regulation, School of Agriculture, Meiji University, 1-1-1, Higashi-Mita, Tama-ku, Kawasaki, Kanagawa, Japan. Electronic address: yoshiya@meiji.ac.jp.
PMID: 36764113 DOI: 10.1016/j.bbrc.2023.01.086
Abstract

DWARF14 (D14) and HTL/KAI2 (KAI2) are paralogous receptors in the α/β-hydrolase superfamily. D14 is the receptor for a class of plant Hormones, strigolactones (SLs), and KAI2 is the receptor for the smoke-derived seed germination inducer, Karrikin (KAR), in Arabidopsis. Germinone (Ger) was previously reported as a KAI2 agonist with germination-inducing activity for thermo-inhibited Arabidopsis seed. However, Ger was not specific to KAI2, and could also bind to D14. It was reported that SL analogs with a desmethyl-type D-ring structure are specifically recognized by KAI2. On the basis of this observation, we synthesized a desmethyl-type germinone (dMGer). We found that dMGer is highly specific to KAI2. Moreover, dMGer induced Arabidopsis seed germination more effectively than did Ger. In addition, dMGer induced the seed germination of Arabidopsis in a manner independently of GA, a well-known germination inducer in Plants.

Keywords

HTL/KAI2; Strigolactone; α/β-hydrolase.

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