2. Academic Validation
  3. The development of nasal polyps involves early middle meatus mucous remodeling via TGF-β1 mediated PAI-1 reduction

The development of nasal polyps involves early middle meatus mucous remodeling via TGF-β1 mediated PAI-1 reduction

  • Braz J Otorhinolaryngol. 2023 Feb 14;S1808-8694(23)00007-1. doi: 10.1016/j.bjorl.2023.01.007.
Yijun Liu 1,  Longlan Shu 1,  Xiaocong Jiang 1,  Yue Zhang 1,  Qian Chen 1,  Yang Shen 1,  Yucheng Yang 2
Affiliations

Affiliations

  • 1 The First Affiliated Hospital of Chongqing Medical University, Department of Otorhinolaryngology, Chongqing, China.
  • 2 The First Affiliated Hospital of Chongqing Medical University, Department of Otorhinolaryngology, Chongqing, China. Electronic address: yychxh@163.com.
PMID: 36841712 DOI: 10.1016/j.bjorl.2023.01.007
Abstract

Objective: Our study aimed to elucidate the effect of PAI-1 (Plasminogen Activator Inhibitor-1) and t-PA (Tissue-type Plasminogen Activator) in tissue remodeling in nasal polyps patients.

Methods: Samples were streamed as early Nasal Polyps (eNP, n=10) and inferior tissue from the same patient, mature Nasal Polyps (mNP, n=14), and Control group (n=15), respectively. Immunohistochemistry and immunofluorescence were applied to detect localization. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) and Western blot were used to measure different levels among three groups. The mNP tissue was cultured in vitro and treated with TGF-β1 (Transforming Growth Factor-beta 1) activator, TGF-β1 inhibitor (SB431542), and PAI-1 Inhibitor (TM5275); then Western blot, qRT-PCR, and ELISA were used to assess changes.

Results: The immunohistochemistry and immunofluorescence showed that PAI-1 expression decreased in eNP and mNP, mainly in epithelium and glands. The transcriptional expression and protein level of TGF-β1/t-PA/PAI-1/Collagen1 were lower in eNP than IT while mNP group demonstrated lower mRNA expression and protein level of TGF-β1/t-PA/PAI-1/Collagen1 than Control group. In mNP tissue culture in vitro, TGF-β1 activator elevated t-PA, PAI-1, and Collagen1 with higher release of PAI-1 and Collagen1 in supernatant, whereas SB431542 suppressed above reactions; TM5275 lowered transcriptional and protein level of Collagen1 in supernatant.

Conclusion: Early Nasal polyps' formation in middle meatus mucous is related with fibrillation system PAI-1/t-PA and tissue remodeling; moreover, nasal polyps' development is regulated by TGF-β1-mediated PAI-1 reduction.

Level of evidence: 3b.

Keywords

Early nasal polyps; Fibrosis; TGF-β1; Tissue culture in vitro; Tissue remodeling.

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