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  3. Protocol to generate induced trophoblast stem cells from embryonic stem cells in mice

Protocol to generate induced trophoblast stem cells from embryonic stem cells in mice

  • STAR Protoc. 2023 Feb 3;4(1):102092. doi: 10.1016/j.xpro.2023.102092.
Yiding Zhao 1,  Qing Wang 1,  Wenhao Zhang 1,  Qingshen Jia 1,  Qian Gao 2,  Ling Shuai 3
Affiliations

Affiliations

  • 1 State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Central Hospital of Gynecology Obstetrics/Tianjin Key Laboratory of Human Development and Reproductive Regulation, Nankai University, Tianjin 300350, China.
  • 2 State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Central Hospital of Gynecology Obstetrics/Tianjin Key Laboratory of Human Development and Reproductive Regulation, Nankai University, Tianjin 300350, China. Electronic address: gaoqian@nankai.edu.cn.
  • 3 State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Central Hospital of Gynecology Obstetrics/Tianjin Key Laboratory of Human Development and Reproductive Regulation, Nankai University, Tianjin 300350, China; Smart Sensing Interdisciplinary Science Center, Nankai University, Tianjin 300350, China. Electronic address: lshuai@nankai.edu.cn.
PMID: 36853659 DOI: 10.1016/j.xpro.2023.102092
Abstract

Conversion of trophectoderm (TE)-derived trophoblast stem cells (TSCs) from inner-cell-mass-derived embryonic stem cells (ESCs) in mice is difficult to achieve naturally. Here, we introduce a reliable and repeatable protocol to generate induced TSCs (iTSCs) from ESCs via a Tet-on system in vitro. The iTSCs show typical TSC properties and have the potential to differentiate into syncytiotrophoblast cells (STCs) and trophoblast giant cells (TGCs). This cell fate transition provides a general platform to robustly investigate the mechanisms underlying TE specification. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).1.

Keywords

Cell Biology; Cell Differentiation; Developmental biology; Flow Cytometry/Mass Cytometry; Gene Expression; Microscopy; Molecular Biology; Stem Cells.

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