2. Academic Validation
  3. Shrimp MANF maintains hemocyte viability via interaction with a tyrosine kinase Abl

Shrimp MANF maintains hemocyte viability via interaction with a tyrosine kinase Abl

  • Dev Comp Immunol. 2023 Feb 28;143:104675. doi: 10.1016/j.dci.2023.104675.
Yaohui Chen 1,  Kaiwen Luo 1,  Baoyuan Zhang 1,  Zhiyao Lu 1,  Fan Wang 2
Affiliations

Affiliations

  • 1 Department of Biology, College of Science, Shantou University, Shantou, 515063, China.
  • 2 Department of Biology, College of Science, Shantou University, Shantou, 515063, China; Institute of Marine Sciences and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, 515063, China; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou, 515063, China. Electronic address: wangfan@stu.edu.cn.
PMID: 36863646 DOI: 10.1016/j.dci.2023.104675
Abstract

Mesencephalic astrocyte-derived neurotrophic factor (MANF) is a highly conserved cell protective protein. In this study, we explored its functions in shrimp hemocytes. Our results indicated that LvMANF knockdown could cause a decrease in total hemocyte count (THC) and an increase in caspase3/7 activity. To further explore its working mechanism, transcriptomic analyses were performed with wild-type and LvMANF-knockdown hemocytes. Three upregulated genes from transcriptomic data, including FAS-associated factor 2, rho-associated protein kinase 1, and serine/threonine-protein kinase WNK4 were validated with qPCR. Further experiments showed that LvMANF knockdown and tyrosine kinase LvAbl knockdown could decrease tyrosine phosphorylation in shrimp hemocytes. In addition, the interaction between LvMANF and LvAbl was validated with immunoprecipitation. The knockdown of LvMANF would decrease ERK phosphorylation and increase LvAbl expression. Our results suggest intracellular LvMANF may maintain shrimp hemocyte viability by interacting with LvAbl.

Keywords

Abl; ERK signaling; MANF.

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