2. Academic Validation
  3. Hypocretin-1 suppresses malignant progression of glioblastoma cells through Notch1 signaling pathway

Hypocretin-1 suppresses malignant progression of glioblastoma cells through Notch1 signaling pathway

  • Brain Res Bull. 2023 Mar 15;196:46-58. doi: 10.1016/j.brainresbull.2023.03.006.
Renzheng Huan 1,  Jianhe Yue 1,  Jinhai Lan 2,  Jia Wang 3,  Yuan Cheng 4,  Jiqin Zhang 5,  Ying Tan 6
Affiliations

Affiliations

  • 1 Department of Neurosurgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
  • 2 Department of the Second Surgery, Ziyun People's Hospital, Anshun, China.
  • 3 Department of Neurosurgery, Chongqing Emergency Medical Center, Chongqing, China.
  • 4 Department of Neurosurgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China. Electronic address: Chengyuan@hospital.cqmu.edu.cn.
  • 5 Department of Anesthesiology, Guizhou Provincial People's Hospital, Guiyang, China. Electronic address: zhangjiqin@gz5055.com.
  • 6 Department of Neurosurgery, Guizhou Provincial People's Hospital, Guiyang, China. Electronic address: tanying@gz5055.com.
PMID: 36925051 DOI: 10.1016/j.brainresbull.2023.03.006
Abstract

Hypocretin-1 is a multifunctional neuropeptide that has been identified as a potential antitumor agent for its role in inhibiting tumor growth, including in colon Cancer, neuroendocrine tumor, and prostate Cancer. However, the role and mechanism of hypocretin-1 in the occurrence and development of malignant glioma have not been well studied. Therefore, we investigated the effect of hypocretin-1 on glioblastoma proliferation, Apoptosis, migration and invasion and its mechanism. We found that the hypocretin-1 receptor was expressed in both glioma cell lines and glioma tissues. Hypocretin-1 treatment can inhibit glioblastoma cell proliferation, migration and invasion, and induce cell Apoptosis. Meanwhile, hypocretin-1 treatment significantly reduces tumor growth rate and tumor weight. In addition, mechanistic studies have found that hypocretin-1 exerts antitumor effects by inhibiting Notch signaling pathway. Overexpression of NICD significantly reversed the antitumor effect of hypocretin on glioblastoma. Taken together, these findings suggest that hypocretin-1 inhibits glioblastoma proliferation, migration and invasion and induces Apoptosis in vitro and in vivo through Notch signaling pathway.

Keywords

Glioblastoma; Hypocretin-1; Malignant progression; Notch signaling pathway.

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