2. Academic Validation
  3. Protocol for capturing trophectoderm stem cells reflecting the blastocyst stage

Protocol for capturing trophectoderm stem cells reflecting the blastocyst stage

  • STAR Protoc. 2023 Mar 16;4(2):102151. doi: 10.1016/j.xpro.2023.102151.
Jinwoo Seong 1,  Nicolas C Rivron 2
Affiliations

Affiliations

  • 1 Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna Biocenter, 1030 Vienna, Austria.
  • 2 Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna Biocenter, 1030 Vienna, Austria. Electronic address: nicolas.rivron@imba.oeaw.ac.at.
PMID: 36930647 DOI: 10.1016/j.xpro.2023.102151
Abstract

Classically, culturing mouse blastocysts with FGF4/TGF-β1, two epiblast-secreted inducers, allows for deriving trophoblast stem cells that comprise fluctuating subpopulations reflecting both pre- and post-implantation stages. However, a more complete combination of inducers (adding LPA, IL11, BMP7, Activin A, 8-Br-cAMP) captures trophectoderm stem cells with enhanced transcriptomic similarity to the blastocyst trophectoderm and self-renewal, reduced differentiation. Also, the complete combination of inducers increased potential to form blastoids and to instruct decidualization in utero, thus better reflecting the blastocyst. For complete details on the use and execution of this protocol, please refer to Seong et al.1.

Keywords

Biotechnology and Bioengineering; Developmental Biology; Stem Cells; Tissue Engineering.

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