Chrysin protects against cerebral ischemia-reperfusion injury in hippocampus via restraining oxidative stress and transition elements

Biomed Pharmacother. 2023 Mar 16;161:114534. doi: 10.1016/j.biopha.2023.114534.

Jinfeng Shang ¹, Jiakang Jiao ², Mingxue Yan ³, Jingyi Wang ⁴, Qiannan Li ⁵, Lizha Shabuerjiang ⁶, Yinghui Lu ⁷, Qi Song ⁸, Lei Bi ⁹, Guijinfeng Huang ¹⁰, Xiaolu Zhang ¹¹, Yinlian Wen ¹², Yiran Cui ¹³, Kai Wu ¹⁴, Gongyu Li ¹⁵, Peng Wang ¹⁶, Xin Liu ¹⁷

Affiliations

1 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China. Electronic address: sjf01qtdd@163.com.
2 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China. Electronic address: jiaojiak123@163.com.
3 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China. Electronic address: 17860506787@163.com.
4 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China. Electronic address: ginyeewang@163.com.
5 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China. Electronic address: liqiannanbucm@163.com.
6 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China. Electronic address: lizasha@163.com.
7 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China. Electronic address: m15001336823@163.com.
8 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China. Electronic address: songqi232@163.com.
9 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China. Electronic address: learn1972@163.com.
10 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China. Electronic address: hgjf2000@163.com.
11 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China. Electronic address: swagdeer@163.com.
12 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China. Electronic address: wenyinlian99@163.com.
13 Department of Pharmacy, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China. Electronic address: cuiyiran@bjzhongyi.com.
14 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China. Electronic address: wukaiLX@163.com.
15 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China. Electronic address: lidoc2727@163.com.
16 Department of Health and Rehabilitation, Beijing College of Sports, Beijing 100075, China. Electronic address: wangtiger6750@126.com.
17 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China. Electronic address: xinliu1011@126.com.

PMID: 36933376 DOI: 10.1016/j.biopha.2023.114534

Abstract

Chrysin is a natural flavonoid compound that has antioxidant and neuroprotective effects. Cerebral ischemia reperfusion (CIR) is closely connected with increased oxidative stress in the hippocampal CA1 region and homeostasis disorder of transition elements such as iron (Fe), copper (Cu) and zinc (Zn). This exploration was conducted to elucidate the antioxidant and neuroprotective effects of chrysin based on transient middle cerebral artery occlusion (tMCAO) in rats. Experimentally, sham group, model group, chrysin (50.0 mg/kg) group, Ginaton (21.6 mg/kg) group, Dimethyloxallyl Glycine (DMOG, 20.0 mg/kg) + chrysin group and DMOG group were devised. The rats in each group were performed to behavioral evaluation, histological staining, biochemical kit detection, and molecular biological detection. The results indicated that chrysin restrained oxidative stress and the rise of transition element levels, and regulated transition element transporter levels in tMCAO rats. DMOG activated hypoxia-inducible factor-1 subunit alpha (HIF-1α), reversed the antioxidant and neuroprotective effects of chrysin, and increased transition element levels. In a word, our findings emphasize that chrysin plays a critical role in protecting CIR injury via inhibiting HIF-1α against enhancive oxidative stress and raised transition metal levels.

Keywords

Cerebral ischemia-reperfusion (CIR); Chrysin; Hippocampus; In vivo; Oxidative stress; Transition element.

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Description

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HY-15893

DMOG

99.69%, HIF-PH Inhibitor

HIF/HIF Prolyl-Hydroxylase; Autophagy

Cancer

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