2. Academic Validation
  3. Strain Streptomyces sp. P-56 Produces Nonactin and Possesses Insecticidal, Acaricidal, Antimicrobial and Plant Growth-Promoting Traits

Strain Streptomyces sp. P-56 Produces Nonactin and Possesses Insecticidal, Acaricidal, Antimicrobial and Plant Growth-Promoting Traits

  • Microorganisms. 2023 Mar 16;11(3):764. doi: 10.3390/microorganisms11030764.
Irina Boykova 1,  Oleg Yuzikhin 1,  Irina Novikova 1,  Pavel Ulianich 2,  Igor Eliseev 3,  Alexander Shaposhnikov 2,  Alexander Yakimov 4,  Andrey Belimov 2
Affiliations

Affiliations

  • 1 All-Russia Institute of Plant Protection, Podbelskogo Sh. 3, Pushkin, Saint-Petersburg 196608, Russia.
  • 2 All-Russia Research Institute for Agricultural Microbiology, Podbelskogo Sh. 3, Pushkin, Saint-Petersburg 196608, Russia.
  • 3 Alferov Federal State Budgetary Institution of Higher Education and Science Saint Petersburg National Research Academic University of the Russian Academy of Sciences, Khlopin Str., 8/3-A, Saint-Petersburg 194021, Russia.
  • 4 Research Center of Nanobiotechnologies, Peter the Great St Petersburg Polytechnic University, Polytechnicheskaya, 29, Saint-Petersburg 195251, Russia.
PMID: 36985337 DOI: 10.3390/microorganisms11030764
Abstract

Streptomycetes produce a huge variety of bioactive metabolites, including Antibiotics, Enzyme inhibitors, pesticides and herbicides, which offer promise for applications in agriculture as plant protection and plant growth-promoting products. The aim of this report was to characterize the biological activities of strain Streptomyces sp. P-56, previously isolated from soil as an insecticidal bacterium. The metabolic complex was obtained from liquid culture of Streptomyces sp. P-56 as dried ethanol extract (DEE) and possessed insecticidal activity against vetch aphid (Medoura viciae Buckt.), cotton aphid (Aphis gossypii Glov.), green peach aphid (Myzus persicae Sulz.), pea aphid (Acyrthosiphon pisum Harr.) and crescent-marked lily aphid (Neomyzus circumflexus Buckt.), as well as two-spotted spider Mite (Tetranychus urticae). Insecticidal activity was associated with production of nonactin, which was purified and identified using HPLC-MS and crystallographic techniques. Strain Streptomyces sp. P-56 also showed Antibacterial and Antifungal activity against various phytopathogenic bacteria and fungi (mostly for Clavibacfer michiganense, Alternaria solani and Sclerotinia libertiana), and possessed a set of plant growth-promoting traits, such as Auxin production, ACC deaminase and phosphate solubilization. The possibilities for using this strain as a biopesticide producer and/or biocontrol and a plant growth-promoting microorganism are discussed.

Keywords

NMR; Streptomyces; X-ray analysis; acaricide; insecticide; nonactin; secondary metabolites; spectral methods.

Figures
Products