Modulation of virus-induced neuroinflammation by the autophagy receptor SHISA9 in mice

Microglia and astrocytes are subgroups of brain glia cells that support and protect neurons within the central nervous system (CNS). At early stages of viral Infection in the CNS, they are predominant responding cells and lead to recruitment of peripheral immune cells for viral clearance. Inhibitor of nuclear factor κB kinase subunit epsilon (IKKi) is critical for type I interferon signalling and inflammation, which modulate heterogenic immune responses during CNS Infection. Balanced Autophagy is vital to maintain brain integrity, yet regulation of Autophagy and immune activity within brain glia cells is poorly understood. Here we identify SHISA9 as an Autophagy cargo receptor that mediates the autophagy-dependent degradation of IKKi during herpes simplex virus type 1 Infection. IKKi is recognized by SHISA9 through unanchored K48-linked poly-ubiquitin chains and bridged to autophagosome membrane components GABARAPL1. Single-cell RNA Sequencing analysis shows that SHISA9 has temporal characteristics while modulating both Antiviral and inflammatory responses in microglia and astrocytes at different stages during viral Infection. We found that Shisa9^-/- mice are highly susceptible to herpes simplex virus encephalitis, have pathogenic astrocytes and display more severe neuroinflammation compared with wild-type mice. Taken together, our study unravels a critical role of selective Autophagy by orchestrating immune heterogeneity of different CNS resident cells through the SHISA9-IKKi axis.