2. Academic Validation
  3. Angelica decursiva exerts antihypertensive activity by inhibiting L-type calcium channel

Angelica decursiva exerts antihypertensive activity by inhibiting L-type calcium channel

  • J Ethnopharmacol. 2023 Sep 15:313:116527. doi: 10.1016/j.jep.2023.116527.
Xiaopei Yang 1,  Chen Na 2,  Yan Wang 3
Affiliations

Affiliations

  • 1 Department of Basic Medicine, Chuxiong Medical College, Chuxiong, 675005, China. Electronic address: xpyang0201@outlook.com.
  • 2 Department of Pediatrics, Yanan Hospital Affiliated to Kunming Medical University, Kunming, Yunnan, 650000, China.
  • 3 Department of Pediatrics, Yanan Hospital Affiliated to Kunming Medical University, Kunming, Yunnan, 650000, China. Electronic address: wangyan740707@163.com.
PMID: 37088236 DOI: 10.1016/j.jep.2023.116527
Abstract

Ethnopharmacological relevance: Angelica decursiva is a perennial herb that belongs to the Umbelliferae family. It is traditionally used to treat fever, upper respiratory tract infections, bleeding and hypertension. However, despite its extensive pharmacological potential, literature reports on its antihypertensive pharmacological properties are scarce.

Aim of the study: In the study, crude extract from A. decursiva roots was examined for its antihypertensive activity and its molecular basis was explored.

Materials and methods: A. decursiva roots were extracted with ethanol, and isolated with silica gel normal-phase chromatography and reverse-phase high performance liquid chromatography. L-NAME-induced hypertensive mouse model was used to detect in vivo hypertensive activity. Thoracic aorta ring contraction activity and electrophysiology recordings were employed to evaluate in vitro antihypertensive activity and revealed an antihypertensive target, which was transiently expressed in HEK293T cells.

Results: Angelica decursiva ethanol decoction (ADED) exhibited significant antihypertensive effects in L-NAME-induced hypertension models and phenylephrine-induced vasoconstriction. Further screening revealed that demethylsuberosin is an essential component accounting for the antihypertension effects of A. decursiva. Voltage-gated Calcium Channel CaV1.2 is the likely target of A. decursiva for its antihypertension effects.

Conclusion: The study suggests that A. decursiva and demethylsuberosin may be effective antihypertensive agents in preclinical studies. It appears that A. decursiva and demethylsuberosin exert antihypertensive effects by inhibiting the CAV1.2 channel, which contributes to the vasodilatory effect. The present study provides experimental evidence that A. decursiva is an effective remedy for hypertension in folklore. Demethylsuberosin could be a lead molecule for antihypertension drug development.

Keywords

Angelica decursiva; Antihypertension; Ca(V)1.2; Demethylsuberosin; Lead molecule.

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