2. Academic Validation
  3. Preventive effect and mechanism of Tibetan tea extract on thrombosis in arachidonic acid-induced zebrafish determined via RNA-seq transcriptome profiles

Preventive effect and mechanism of Tibetan tea extract on thrombosis in arachidonic acid-induced zebrafish determined via RNA-seq transcriptome profiles

  • PLoS One. 2023 May 19;18(5):e0285216. doi: 10.1371/journal.pone.0285216.
Ning Wang 1 2 3 4,  Chaohua Lan 1,  Huiqiang Lu 5,  Linman Li 1,  Dalong Liao 1,  Kewei Xu 1,  Haiyan Sun 4,  Yongqing Tang 4,  Yumeng Wang 6,  Jie Mei 7,  Mengting Wei 1,  Tao Wu 8,  Hui Zhu 1
Affiliations

Affiliations

  • 1 College of Bioengineering, Sichuan University of Science and Engineering, Zigong, China.
  • 2 Luzhou Laojiao Co. Ltd, Luzhou, PR China.
  • 3 College of Horticulture, Hunan Agricultural University, Changsha, China.
  • 4 Chengdu Chongqing Shuangcheng Economic Circle (Luzhou) Advanced Technology Research Institute, Luzhou, China.
  • 5 Center for Drug Screening and Research, School of Geography and Environmental Engineering, Gannan Normal University, Ganzhou, China.
  • 6 Bristol Myers Squibb, Princeton, NJ, United States of America.
  • 7 Sichuan Jixiang Tea Co., Ltd., Ya'an, China.
  • 8 School of Food and Biological Engineering, Xihua University, Chengdu, China.
PMID: 37205684 DOI: 10.1371/journal.pone.0285216
Abstract

Thrombosis is a key pathological event in cardiovascular diseases and is also the most important targeting process for their clinical management. In this study, arachidonic acid (AA) was used to induce thrombus formation in zebrafish larvae. Blood flow, red blood cell (RBCs) aggregation and cellular oxidative stress were measured to evaluate the antithrombotic effect of Tibetan tea (TT). Meanwhile, the potential molecular mechanism was further explored by transcriptome Sequencing (RNA-seq). The results indicated that TT could significantly restore heart RBCs intensity of thrombotic zebrafish, whilst decreasing RBCs accumulation in the caudal vein. The transcriptome analysis revealed that the preventive effect of TT on thrombosis could be mostly attributed to changes in lipid metabolism related signaling pathways, such as fatty acid metabolism, glycerollipid metabolism, ECM-receptor interaction and steroid biosynthesis signaling pathway. This study demonstrated that Tibetan tea could alleviate thrombosis by reducing oxidative stress levels and regulating lipid metabolism.

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