2. Academic Validation
  3. 5-ALA-PDT induced ferroptosis in keloid fibroblasts via ROS, accompanied by downregulation of xCT, GPX4

5-ALA-PDT induced ferroptosis in keloid fibroblasts via ROS, accompanied by downregulation of xCT, GPX4

  • Photodiagnosis Photodyn Ther. 2023 May 21;103612. doi: 10.1016/j.pdpdt.2023.103612.
Jiheng Zhang 1,  Lulu Liu 1,  Xinying Li 1,  Xiaoxiao Shen 2,  Guihong Yang 3,  Yumeng Deng 4,  Zhengwei Hu 5,  Junbo Zhang 6,  Yuangang Lu 7
Affiliations

Affiliations

  • 1 Department of Plastic and Cosmetic Surgery, Daping Hospital, Army Medical University, Chongqing, China.
  • 2 Bioengineering College of Chongqing University, Chongqing, China.
  • 3 Department of Dermatology, Daping Hospital, Army Medical University, Chongqing, China.
  • 4 Department of Dermatology, Third Affiliated Hospital of Chongqing Medical University, Chongqing, China.
  • 5 Department of Urology, Daping Hospital, Army Medical University, Chongqing, China.
  • 6 Department of Plastic and Cosmetic Surgery, Daping Hospital, Army Medical University, Chongqing, China. Electronic address: zhangjunbo0924@163.com.
  • 7 Department of Plastic and Cosmetic Surgery, Daping Hospital, Army Medical University, Chongqing, China. Electronic address: skin515@163.com.
PMID: 37220842 DOI: 10.1016/j.pdpdt.2023.103612
Abstract

Keloids display many cancerous properties, including uncontrolled and invasive growth, high rates of recurrence as well as similar bioenergetics. 5-aminolevulinic acid-based photodynamic therapy (5-ALA-PDT) is an effective treatment that performs cytotoxic effects by producing Reactive Oxygen Species (ROS), which is linked to lipid peroxidation and Ferroptosis. Herein, we explored underlying mechanisms of 5-ALA-PDT against keloids. We identified that 5-ALA-PDT led to elevated levels of ROS and lipid peroxidation in keloid fibroblasts, accompanied by downregulation of xCT and GPX4, which are associated with anti-oxidation effects and Ferroptosis inhibition. These results may indicate that 5-ALA-PDT treatment increases ROS while inhibiting xCT and GPX4, thus promoting lipid peroxidation to induce Ferroptosis in keloid fibroblasts.

Keywords

PDT; ferroptosis; fibroblast; keloids.

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