Sufentanil inhibits Pin1 to attenuate renal tubular epithelial cell ischemia-reperfusion injury by activating the PI3K/AKT/FOXO1 pathway

Background: Renal ischemia-reperfusion injury (RIRI) has become a great concern in clinical practice with high morbidity and mortality rates. Sufentanil has protective effects on IRI-induced organ injury. Herein, the effects of sufentanil on RIRI were investigated.

Methods: RIRI cell model was established by hypoxia/reperfusion (H/R) stimulation. The mRNA and protein expressions were assessed using qRT-PCR and western blot. TMCK-1 cell viability and Apoptosis were assessed using MTT assay and flow cytometry, respectively. The mitochondrial membrane potential and ROS level were detected by JC-1 mitochondrial membrane potential fluorescent probe and DCFH-DA fluorescent probe, respectively. LDH, SOD, CAT, GSH and MDA levels were determined by the kits. The interaction between FOXO1 and PIN1 promoter was analyzed using dual luciferase reporter gene and ChIP assays.

Results: Our results revealed that sufentanil treatment attenuated H/R-induced cell Apoptosis, mitochondrial membrane potential (MMP) dysfunction, oxidative stress, inflammation and activated PI3K/Akt/FOXO1 associated proteins, while these effects were reversed by PI3K Inhibitor, suggesting that sufentanil attenuated RIRI via activating the PI3K/Akt/FOXO1 signaling pathway. We subsequently found that FOXO1 transcriptionally activated PIN1 in TCMK-1 cells. PIN1 inhibition ameliorated H/R-induced TCMK-1 cell Apoptosis, oxidative stress and inflammation. In addition, as expected, the biological effects of sufentanil on H/R-treated TMCK-1 cells were abrogated by PIN1 overexpression.

Conclusion: Sufentanil reduced PIN1 expression through activation of the PI3K/Akt/FOXO1 signaling to suppress cell Apoptosis, oxidative stress and inflammation in renal tubular epithelial cells during RIRI development.

Inflammation; Oxidative stress; Pin1; RIRI; Sufentanil.