1. Academic Validation
  2. Lycopene abolishes typical polyhalogenated carbazoles (PHCZs)-induced hepatic injury in yellow catfish (Pelteobagrus fulvidraco): Involvement of ROS/PI3K-AKT/NF-κB signaling

Lycopene abolishes typical polyhalogenated carbazoles (PHCZs)-induced hepatic injury in yellow catfish (Pelteobagrus fulvidraco): Involvement of ROS/PI3K-AKT/NF-κB signaling

  • Fish Shellfish Immunol. 2023 Jun 8;108897. doi: 10.1016/j.fsi.2023.108897.
Siwen Li 1 Jiaqi Xie 2 Dongfang Zhang 3 Guifang Zhao 3 Yiang Bai 1 Keman Li 1 Xinlian Li 4 Qiuyue Li 4 Xiaoqing Tang 4 Xiaofeng Ge 5
Affiliations

Affiliations

  • 1 Xiangya School of Public Health, Central South University, Changsha, 410078, Hunan Province, PR China.
  • 2 Hunan Food and Drug Vocational College, Changsha, 410078, Hunan Province, PR China.
  • 3 Department of Pathology, Jilin Medical University, Jilin, 130013, Jilin Province, PR China.
  • 4 Department of Physiology, College of Basic Medical Sciences, Southwest Medical University, Luzhou, 646000, Sichuan Province, PR China.
  • 5 Jilin People's Hospital, Jilin, 130013, Jilin Province, PR China. Electronic address: 18104422692@163.com.
Abstract

Aquatic ecosystems are being more contaminated with polyhalogenated carbazoles (PHCZs), which raising concerns about their impact on aquatic organisms. Lycopene (LYC) exhibits several beneficial properties for fish via enhance antioxidant defenses and improve immunity. In this study, we attempted to investigate the hepatotoxic effects of typical PHCZs 3, 6-dichlorocarbazole (3,6-DCCZ) and the protective mechanisms of LYC. In this study, we found that yellow catfish (Pelteobagrus fulvidraco) exposure to 3,6-DCCZ (1.2 mg/L) resulted in hepatic inflammatory infiltration and disordered hepatocyte arrangement. Besides, we observed that 3,6-DCCZ exposure resulted in hepatic Reactive Oxygen Species (ROS) overproduction and excessive autophagosome accumulation, accompanied with inhibition of phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt) pathway. Subsequently, we confirmed that 3,6-DCCZ exposure triggered hepatic uncontrolled inflammatory response via activation of nuclear factor-κB (NF-κB) pathway, along with decreased plasma complement C3 (C3) and complement C4 (C4) levels. Meanwhile, yellow catfish exposed to 3,6-DCCZ exhibit an increased hepatic Apoptosis phenomenon, as evidenced by the elevated number of positive TUNEL cells and upregulated expression of caspase3 and cytochrome C (CytC). In contrast, LYC treatment could alleviate the 3,6-DCCZ-induced pathological changes, hepatic ROS accumulation, Autophagy, inflammatory response and Apoptosis. To sum up, this study provided the demonstration that LYC exerts hepatoprotective effects to alleviate 3,6-DCCZ-induced liver damage by inihibiting ROS/PI3K-AKT/NF-κB signaling in yellow catfish.

Keywords

Autophagy; Inflammatory response; Liver damage; Polyhalogenated carbazoles (PHCZs); Reactive oxygen species (ROS) accumulation.

Figures
Products