2. Academic Validation
  3. Short-term in vitro exposure of Pinctada imbricata's haemocytes to quaternium-15: Exploring physiological and cellular responses

Short-term in vitro exposure of Pinctada imbricata's haemocytes to quaternium-15: Exploring physiological and cellular responses

  • Environ Toxicol Pharmacol. 2023 Aug:101:104198. doi: 10.1016/j.etap.2023.104198.
Cristiana Roberta Multisanti 1,  Kristian Riolo 1,  Federica Impellitteri 2,  Imen Chebbi 3,  Caterina Faggio 4,  Alessia Giannetto 1
Affiliations

Affiliations

  • 1 Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno D'Alcontres, 31, 98166 Messina, Italy.
  • 2 Department of Veterinary Sciences, University of Messina, Viale Giovanni Palatucci snc, 98168 Messina, Italy.
  • 3 Laboratory of Biodiversity and Aquatic Ecosystems, Faculty of Science, University of Sfax, BP, 3038, Tunisia.
  • 4 Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno D'Alcontres, 31, 98166 Messina, Italy. Electronic address: cfaggio@unime.it.
PMID: 37391050 DOI: 10.1016/j.etap.2023.104198
Abstract

Since the 2000 s, the pearl oyster Pinctada imbricata (Röding, 1798) has become established along the transitional waterways of the "Capo Peloro Lagoon" natural reserve, where it is now abundant due to its adaptability to different hydrological, climatic, environmental, and pollution conditions. This study aims to evaluate haemocyte immune-mediated responses in vitro to quaternium-15, a common pollutant in aquatic ecosystems. Cell viability and phagocytosis activity decreased when exposed to 0.1 or 1 mg L- 1 of quaternium-15. Moreover, decreasing phagocytosis was confirmed by gene expression modulation of actin, involved in Cytoskeleton rearrangement. Effects on oxidative stress-related genes were also assessed (Cat, MnSod, Zn/CuSod, GPx). The qPCR data revealed alterations in antioxidant responses through gene dose- and time-dependent modulation. This study presents insights into the physiological responses and cellular mechanisms of P. imbricata haemocytes to environmental stressors, indicating that this species is useful as a novel bioindicator for future toxicological studies.

Keywords

Gene expression; Phagocytosis; Pinctada imbricata; Shellfish immunology.

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