2. Academic Validation
  3. Selenomethionine Inhibits NF-κB-mediated Inflammatory Responses of Bovine Mammary Epithelial Cells Caused by Klebsiella pneumoniae by Increasing Autophagic Flux

Selenomethionine Inhibits NF-κB-mediated Inflammatory Responses of Bovine Mammary Epithelial Cells Caused by Klebsiella pneumoniae by Increasing Autophagic Flux

  • Biol Trace Elem Res. 2023 Jul 5. doi: 10.1007/s12011-023-03757-2.
Siyan Xu 1 2 3 4,  Yixue Miao 1 2 3 4,  Junsheng Dong 1 2 3 4,  Luying Cui 1 2 3 4,  Kangjun Liu 1 2 3 4,  Jianji Li 1 2 3 4,  Xia Meng 1 2 3,  Guoqiang Zhu 1 2 3,  Heng Wang 5 6 7 8
Affiliations

Affiliations

  • 1 College of Veterinary Medicine, Yangzhou University, 225009, Yangzhou, China.
  • 2 Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, Jiangsu, China.
  • 3 International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of Jiangsu Higher Education Institutions, Yangzhou University, Yangzhou, 225009, Jiangsu, China.
  • 4 Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou, 225009, Jiangsu, China.
  • 5 College of Veterinary Medicine, Yangzhou University, 225009, Yangzhou, China. sdaulellow@163.com.
  • 6 Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, Jiangsu, China. sdaulellow@163.com.
  • 7 International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of Jiangsu Higher Education Institutions, Yangzhou University, Yangzhou, 225009, Jiangsu, China. sdaulellow@163.com.
  • 8 Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou, 225009, Jiangsu, China. sdaulellow@163.com.
PMID: 37407885 DOI: 10.1007/s12011-023-03757-2
Abstract

Klebsiella pneumoniae (K. pneumoniae) is one of the major pathogens causing bovine clinical mastitis. Autophagy maintains cellular homeostasis and resists excessive inflammation in eukaryotic organisms. Selenomethionine (Se-Met) is commonly used as a source of selenium supplementation for dairy cows. This study aimed to investigate the effects of Se-Met on inflammatory responses mediated by nuclear factor-kappa B (NF-κB) through Autophagy. We infected bovine mammary epithelial cell line (MAC-T) with K. pneumoniae and examined the expression of autophagy-related proteins and changes in autophagic vesicles, LC3 puncta, and autophagic flux at various intervals. The results showed that K. pneumoniae activated the early-stage Autophagy of MAC-T cells. The levels of LC3-II, Beclin1, and ATG5, as well as the number of LC3 puncta and autophagic vesicles, increased after 2 h post-treatment. However, the late-stage autophagic flux was blocked. Furthermore, the effect of Autophagy on NF-κB-mediated inflammation was investigated with different Autophagy levels. The findings showed that enhanced Autophagy inhibited the K. pneumoniae-induced inflammatory responses of MAC-T cells. The opposite results were found with the inhibition of Autophagy. Finally, we examined the effect of Se-Met on NF-κB-mediated inflammation based on Autophagy. The results indicated that Se-Met alleviated K. pneumoniae-induced autophagic flux blockage, inhibited NF-κB-mediated inflammation, and decreased the adhesion of K. pneumoniae to MAC-T cells. The inhibitory effect of Se-Met on NF-κB-mediated inflammation could be partially blocked by the Autophagy Inhibitor chloroquine (CQ). Overall, Se-Met attenuated K. pneumoniae-induced NF-κB-mediated inflammatory responses by enhancing autophagic flux.

Keywords

Autophagy; Bovine mammary epithelial cells; Inflammatory responses; K. pneumoniae; Selenomethionine.

Figures
Products