2. Academic Validation
  3. Inhibition of CARM1 suppresses proliferation of multiple myeloma cells through activation of p53 signaling pathway

Inhibition of CARM1 suppresses proliferation of multiple myeloma cells through activation of p53 signaling pathway

  • Mol Biol Rep. 2023 Jul 21. doi: 10.1007/s11033-023-08645-5.
Lan Yang # 1,  Le Ma # 2,  Qiang Gong 3,  JiePing Chen 4,  Qilin Huang 5 6
Affiliations

Affiliations

  • 1 Medical College of Guizhou University, Guiyang City, 550025, China.
  • 2 Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Army Medical University), Third Military Medical University, Chongqing, 400038, China.
  • 3 Department of Hematology, Southwest Hospital, Third Military Medical University (Army Medical University), Gaotanyan Road Street, Shapingba District, 400038, China. gongqiang_cool@163.com.
  • 4 Department of Hematology, Southwest Hospital, Third Military Medical University (Army Medical University), Gaotanyan Road Street, Shapingba District, 400038, China. chenjpxn@163.com.
  • 5 Medical College of Guizhou University, Guiyang City, 550025, China. hqlxqyy@sina.com.
  • 6 Department of Neurosurgery, Guiqian International General Hospital, Changpo Road, Wudang District, Guiyang City, 550000, China. hqlxqyy@sina.com.
  • # Contributed equally.
PMID: 37477799 DOI: 10.1007/s11033-023-08645-5
Abstract

Background: Multiple myeloma (MM) is a malignant proliferative disease of plasma cells, the incidence of which is increasing every year and remains incurable. The Enzyme co-activator-associated arginine methyltransferase 1 (CARM1) is highly expressed in a variety of cancers, such as Hodgkin's lymphoma and acute myeloid leukemia, and CARM1 is closely associated with tumor cell proliferation. However, the role of CARM1 in MM has not been elucidated.

Methods and results: In this study, we found that CARM1 is overexpressed in MM and closely associated with poor prognosis in MM. CCK-8 and colony formation assays showed that the proliferation of MM cell lines was downregulated when CARM1 expression was knockdown by specific shRNA. Knockdown of CARM1 reduced the proportion of MM cell lines in the S phase and increased the proportion in G0/G1 phase. RNA-seq analysis of the CARM1-KD cell line revealed that it was closely associated with Apoptosis and activated the p53 pathway. CCK-8 and Apoptosis results showed that CARM1 knockdown made MM cells more sensitive to standard-of-care drugs.

Conclusion: This study provides an experimental basis for elucidating the pathogenesis of multiple myeloma and searching for potential therapeutic targets.

Keywords

CARM1; Cell proliferation; Multiple myeloma; p53.

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