2. Academic Validation
  3. Taxonomy and anticancer potential of Streptomyces niphimycinicus sp. nov. against nasopharyngeal carcinoma cells

Taxonomy and anticancer potential of Streptomyces niphimycinicus sp. nov. against nasopharyngeal carcinoma cells

  • Appl Microbiol Biotechnol. 2023 Aug 11. doi: 10.1007/s00253-023-12707-w.
Yiying Huang 1 2 3,  Wenjin Hu 4 5,  Shushi Huang 4,  Jiemei Chu 6,  Yushan Liang 1,  Zhanhua Tao 4,  Guiwen Wang 4,  Junlian Zhuang 4,  Zhe Zhang 7 8 9,  Xiaoying Zhou 10 11 12,  Xinli Pan 13
Affiliations

Affiliations

  • 1 Department of Otolaryngology-Head and Neck Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.
  • 2 Key Laboratory of Early Prevention and Treatment for Regional High-Frequency Tumor (Guangxi Medical University), Ministry of Education, Nanning, China.
  • 3 Guangxi Key Laboratory of Early Prevention and Treatment for Regional High-Frequency Tumor, Nanning, China.
  • 4 Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Institute of Eco-Environmental Research, Guangxi Academy of Sciences, Nanning, China.
  • 5 National Engineering Research Center for Non-Food Biorefinery, State Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Key Laboratory of Biorefinery, Guangxi Biomass Engineering Technology Research Center, Guangxi Academy of Sciences, Nanning, China.
  • 6 Life Science Institute, Guangxi Medical University, Nanning, China.
  • 7 Department of Otolaryngology-Head and Neck Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, China. zhangzhe1975@gmail.com.
  • 8 Key Laboratory of Early Prevention and Treatment for Regional High-Frequency Tumor (Guangxi Medical University), Ministry of Education, Nanning, China. zhangzhe1975@gmail.com.
  • 9 Guangxi Key Laboratory of Early Prevention and Treatment for Regional High-Frequency Tumor, Nanning, China. zhangzhe1975@gmail.com.
  • 10 Life Science Institute, Guangxi Medical University, Nanning, China. zhouxiaoying1982@foxmail.com.
  • 11 Key Laboratory of Early Prevention and Treatment for Regional High-Frequency Tumor (Guangxi Medical University), Ministry of Education, Nanning, China. zhouxiaoying1982@foxmail.com.
  • 12 Guangxi Key Laboratory of Early Prevention and Treatment for Regional High-Frequency Tumor, Nanning, China. zhouxiaoying1982@foxmail.com.
  • 13 Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Institute of Eco-Environmental Research, Guangxi Academy of Sciences, Nanning, China. pan_xin_li@163.com.
PMID: 37566161 DOI: 10.1007/s00253-023-12707-w
Abstract

Streptomyces species are ubiquitous, Gram-positive, spore-forming bacteria with the ability to produce various clinically relevant compounds. The strain 4503 T was isolated from mangrove sediments, showing morphological and chemical properties which were consistent with those of members of the genus Streptomyces. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate was primarily identified as members of the genus Streptomyces, sharing more than 99% sequence identity to Streptomyces yatensis DSM 41771 T, S. antimycoticus NBRC 12839 T, and S. melanosporofaciens NBRC 13061 T. Average nucleotide identities (ANI) and digital DNA-DNA hybridization (dDDH) values between strain 4503 T and its close relatives were all below 95-96% and 75% of the novel species threshold, respectively. Results from phylogenetic, genomic, phenotypic, and chemotaxonomic characteristics analyses confirmed that the isolate represented a novel species of the genus Streptomyces, for which the name Streptomyces niphimycinicus sp. nov. 4503 T (= MCCC 1K04557T = JCM 34996 T) is proposed. The bioassay-guided fractionation of the extract of strain 4503 T resulted in the isolation of a known compound niphimycin C, which showed cytotoxic activity against nasopharyngeal carcinoma (NPC) cell lines TW03 and 5-8F with half maximal inhibitory concentration (IC50) values of 12.24 µg/mL and 9.44 µg/mL, respectively. Further experiments revealed that niphimycin C not only exhibited the capacity of anti-proliferation, anti-metastasis, induction of cell cycle arrest, and Apoptosis, but was also able to increase the Reactive Oxygen Species (ROS) production and regulate several signaling pathways in NPC cells. KEY POINTS: • Strain 4503 T was classified as a novel species of Streptomyces. • Niphimycin C correlates with the cytotoxic effect of strain 4503 T against NPC cells. • Niphimycin C induces Apoptosis, autophagic flux disruption and cell cycle arrest.

Keywords

Mangrove; Nasopharyngeal carcinoma; Niphimycin C; Streptomyces.

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