2. Academic Validation
  3. Exosomes derived from ovarian cancer cells regulate proliferation and migration of cancer-associated fibroblasts

Exosomes derived from ovarian cancer cells regulate proliferation and migration of cancer-associated fibroblasts

  • Genomics. 2023 Sep;115(5):110703. doi: 10.1016/j.ygeno.2023.110703.
Bo Ding 1,  Zheng Ye 2,  Han Yin 1,  Xin-Yi Hong 1,  Song-Wei Feng 1,  Jing-Yun Xu 1,  Yang Shen 3
Affiliations

Affiliations

  • 1 Department of Obstetrics and Gynecology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, China.
  • 2 State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China.
  • 3 Department of Obstetrics and Gynecology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, China. Electronic address: shenyang@seu.edu.cn.
PMID: 37678440 DOI: 10.1016/j.ygeno.2023.110703
Abstract

Cancer-associated fibroblast (CAF) is an essential risk factor for ovarian Cancer. Exosomes can mediate cellular communication in the tumour microenvironment, but the interaction of tumour cell exosomes with CAF is less studied in Ovarian Cancer. This study identified H19/miR-29c-3p/LOXL2-COL1A1 as a ceRNA regulatory network involved in regulating tumour matrix-associated signaling pathways associated with CAF. Cellular assays demonstrated that exosomes from ovarian Cancer cell line SKOV3 significantly promoted the proliferation and migration of CAF. The results of mixed transplantation tumour experiments in nude mice showed that exosomes of SKOV3 significantly promoted tumour growth. Ovarian Cancer tumour-derived exosomes can regulate CAF proliferation and migration through H19/miR-29c-3p/LOXL2-COL1A1. This study reveals the regulatory role of tumour exosomes on CAF, which may provide a theoretical basis for the development of therapeutic regimens targeting fibroblasts in ovarian Cancer.

Keywords

CAF; H19; Tumour microenvironment; WGCNA; ceRNA; miR-29c-3p.

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