2. Academic Validation
  3. Tanshinone I attenuates gastric precancerous lesions by inhibiting epithelial mesenchymal transition through the p38/STAT3 pathway

Tanshinone I attenuates gastric precancerous lesions by inhibiting epithelial mesenchymal transition through the p38/STAT3 pathway

  • Int Immunopharmacol. 2023 Sep 10;124(Pt A):110902. doi: 10.1016/j.intimp.2023.110902.
Dan Liang 1,  Shiyun Tang 1,  Lu Liu 2,  Maoyuan Zhao 1,  Xiao Ma 2,  Yanling Zhao 3,  Caifei Shen 4,  Qingsong Liu 5,  Jianyuan Tang 6,  Jinhao Zeng 7,  Nianzhi Chen 8
Affiliations

Affiliations

  • 1 Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.
  • 2 College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
  • 3 Department of Pharmacy, Chinese PLA General Hospital, Beijing, China.
  • 4 Department of Endoscopy Center, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.
  • 5 Department of Gastroenterology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.
  • 6 TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China. Electronic address: tangjy@cdutcm.edu.cn.
  • 7 Department of Gastroenterology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China. Electronic address: zengjinhao@cdutcm.edu.cn.
  • 8 State Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing, China. Electronic address: saber930607@163.com.
PMID: 37699302 DOI: 10.1016/j.intimp.2023.110902
Abstract

Background: Gastric precancerous lesions (GPLs) are omens for gastric Cancer (GC), which developing with a series of pathological changes of gastric mucosa. Reversing epithelial-mesenchymal transition (EMT) in gastric mucosa is the main approach to restrain GPLs from evolving into Cancer. Tanshinone I (Tan-I), the active ingredients of traditional Chinese herb Salvia miltiorrhiza, has exhibited Anticancer effect.

Purpose: To investigate the effect and mechanism of Tan-I in intervening GPLs, and provide a new therapeutic strategy for prevention of GC.

Methods: Gastric mucosal epithelial cells were treated with the N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) to construct MNNG-induced cell (MC cell) of gastric mucosa that undergoing EMT process. Then, this study explored the effect and mechanism of Tan-I in vitro. Subsequently, this study constructed GPL mice to clarify the exact efficacy and mechanism of Tan-I on GPLs.

Results: Tan-I inhibited MC cell proliferation, invasion and migration. Simultaneously, the aberrant expression of E-cadherin and N-Cadherin were reversed. Tan-I attenuated inflammation by reducing the release of nitric oxide, TNFα and IL-1β. Tan-I reversed the EMT and inflammatory processes by regulating p38 and STAT3.

Conclusion: This study showed that Tan-I inhibited the progression of GPLs by reversing the EMT process and reducing inflammation by restraining the p38/STAT3 signaling pathway.

Keywords

Epithelial-mesenchymal transition; Gastric cancer; Gastric precancerous lesions; Tanshinone I; p38/STAT3.

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