2. Academic Validation
  3. Inhibition of STIM1 alleviates high glucose-induced proliferation and fibrosis by inducing autophagy in mesangial cells

Inhibition of STIM1 alleviates high glucose-induced proliferation and fibrosis by inducing autophagy in mesangial cells

  • Mol Cell Biochem. 2023 Sep 22. doi: 10.1007/s11010-023-04844-7.
Xixi Zeng 1,  Anbang Sun 1,  Weiyi Cheng 2,  Xin Hou 3,  Min Zhu 4,  Yanhong Liao 5
Affiliations

Affiliations

  • 1 Department of Anatomy, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, 430030, Hubei, People's Republic of China.
  • 2 Department of Emergency Surgery, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, 430030, Hubei, People's Republic of China.
  • 3 Medical College, Affiliated Hospital, Hebei University of Engineering, Handan, People's Republic of China.
  • 4 Department of Thoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China. mzhu@tjh.tjmu.edu.cn.
  • 5 Department of Anatomy, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, 430030, Hubei, People's Republic of China. yhliao1@hust.edu.cn.
PMID: 37736800 DOI: 10.1007/s11010-023-04844-7
Abstract

Diabetic nephropathy (DN) is a renal microvascular complication caused by diabetes mellitus. One of the most typical characteristics of DN is glomerular mesangial cells (GMCs) proliferation. Stromal interaction molecule 1 (STIM1), a CA2+ channel, is involved in many diseases. In this study, we investigated the role of STIM1 in the proliferation and fibrosis in high glucose (HG)-induced HBZY-1 cells. We found that the expression of STIM1 was increased in renal tissues of diabetic rat and HBZY-1 cells stimulated by HG. Downregulation of STIM1-mediated SOCE suppressed hyperglycemic cell proliferation and fibrosis by activating Autophagy. In addition, the inhibitory effect of downregulating STIM1 on cells was blocked by Autophagy Inhibitor Bafilomycin A1 (BafA1). Moreover, this experiment also showed that STIM1 regulated Autophagy, cell proliferation and fibrosis via PI3K/Akt/mTOR signal pathway. These results clarify the role of STIM1 in HBZY-1 cells and its mechanism, and provide a new target for the treatment of DN.

Keywords

Autophagy; Diabetic nephropathy; Fibrosis; Proliferation; SOCE; STIM1.

Figures
Products