2. Academic Validation
  3. Improved production of andrimid in Erwinia persicina BST187 strain by fermentation optimization

Improved production of andrimid in Erwinia persicina BST187 strain by fermentation optimization

  • BMC Microbiol. 2023 Sep 25;23(1):268. doi: 10.1186/s12866-023-02946-2.
Tingfeng Cheng 1 2,  Tongling Ge 1 3,  Lunqiang Zhao 1 4,  Yuyong Hou 1 3,  Jianye Xia 5 6,  Lei Zhao 7 8 9
Affiliations

Affiliations

  • 1 Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, China.
  • 2 University of Chinese Academy of Sciences, Beijing, China.
  • 3 National Center of Technology Innovation for Synthetic Biology, Tianjin, China.
  • 4 College of Biological Sciences, China Agricultural University, Beijing, China.
  • 5 Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, China. xiajy@tib.cas.cn.
  • 6 National Center of Technology Innovation for Synthetic Biology, Tianjin, China. xiajy@tib.cas.cn.
  • 7 Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, China. zhaol@tib.cas.cn.
  • 8 National Center of Technology Innovation for Synthetic Biology, Tianjin, China. zhaol@tib.cas.cn.
  • 9 College of Biological Sciences, China Agricultural University, Beijing, China. zhaol@tib.cas.cn.
PMID: 37749510 DOI: 10.1186/s12866-023-02946-2
Abstract

Background: Andrimid is reported to be a novel kind of polyketide-nonribosomal peptide hybrid product (PK-NRPs) that inhibits fatty acid biosynthesis in bacteria. Considering its great potential in biomedicine and biofarming, intensive studies have been conducted to increase the production of andrimid to overcome the excessive costs of chemosynthesis. In screening for species with broad-spectrum Antibacterial activity, we detected andrimid in the fermentation products of Erwinia persicina BST187. To increase andrimid production, the BST187 fermentation medium formulation and fermentation conditions were optimized by using systematic design of experiments (One-Factor-At-A-Time, Plackett-Burman design, Response Surface Methodology).

Results: The results indicate that the actual andrimid production reached 140.3 ± 1.28 mg/L under the optimized conditions (trisodium citrate dihydrate-30 g/L, beef extract-17.1 g/L, MgCl2·6H2O-100 mM, inoculation amount-1%, initial pH-7.0, fermentation time-36 h, temperature-19.7℃), which is 20-fold greater than the initial condition without optimization (7.00 ± 0.40 mg/L), consistent with the improved Antibacterial effect of the fermentation supernatant.

Conclusions: The present study provides valuable information for improving andrimid production via optimization of the fermentation process, which will be of great value in the future industrialization of andrimid production.

Keywords

Andrimid; Antibacterial activity; Design of experiment; Erwinia persicina; Fermentation; Response surface methodology.

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