2. Academic Validation
  3. Multifunctional CaCO3@Cur@QTX125@HA nanoparticles for effectively inhibiting growth of colorectal cancer cells

Multifunctional CaCO3@Cur@QTX125@HA nanoparticles for effectively inhibiting growth of colorectal cancer cells

  • J Nanobiotechnology. 2023 Sep 29;21(1):353. doi: 10.1186/s12951-023-02104-w.
Shengyun Hu # 1,  Kunkun Xia # 1,  Xiaobei Huang # 2,  Ye Zhao 3,  Qingqing Zhang 1,  Dongdong Huang 4,  Weiyi Xu 5,  Zhengju Chen 6,  Chenfei Wang 7,  Zhiyong Zhang 8
Affiliations

Affiliations

  • 1 Department of Colorectal and Anal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
  • 2 Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714, China.
  • 3 Department of Gastroenterology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
  • 4 Pooling Medical Research Institutes of 100Biotech, Beijing, 100006, China.
  • 5 Department of Dermatology, National Children's Medical Center, Children's Hospital of Fudan University, Shanghai, 201102, China.
  • 6 Pooling Medical Research Institutes of 100Biotech, Beijing, 100006, China. melody@100biotech.com.
  • 7 Department of Dermatology, National Children's Medical Center, Children's Hospital of Fudan University, Shanghai, 201102, China. wangchenfei@fudan.edu.cn.
  • 8 Department of Colorectal and Anal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. fcczhangzy2@zzu.edu.cn.
  • # Contributed equally.
PMID: 37773145 DOI: 10.1186/s12951-023-02104-w
Abstract

Colorectal Cancer (CRC) is a major cause of cancer-related deaths in humans, and effective treatments are still needed in clinical practice. Despite significant developments in Anticancer drugs and inhibitors, their poor stability, water solubility, and cellular membrane permeability limit their therapeutic efficacy. To address these issues, multifunctional CaCO3 nanoparticles loaded with Curcumin (Cur) and protein deacetylase (HDAC) inhibitor QTX125, and coated with hyaluronic acid (HA) (CaCO3@Cur@QTX125@HA), were prepared through a one-step gas diffusion strategy. Dynamic LIGHT scattering (DLS), transmission electron microscopy (TEM), and scanning electron microscopy (SEM) showed that CaCO3@Cur@QTX125@HA nanoparticles have uniform spherical morphology and elemental distribution, with diameters around 450 nm and a Zeta potential of - 8.11 mV. The controlled release of Cur from the nanoparticles was observed over time periods of 48 h. Cellular uptake showed that CaCO3@Cur@QTX125@HA nanoparticles were efficiently taken up by Cancer cells and significantly inhibited their growth. Importantly, CaCO3@Cur@QTX125@HA nanoparticles showed specific inhibitory effects on CRC cell growth. Encouragingly, CaCO3@Cur@QTX125@HA nanoparticles successfully internalized into CRC patient-derived Organoid (PDO) models and induced Apoptosis of tumor cells. The multifunctional CaCO3@Cur@QTX125@HA nanoparticles hold promise for the treatment of CRC.

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