2. Academic Validation
  3. Polydatin improves vascular endothelial function by maintaining mitochondrial homeostasis under high glucose conditions

Polydatin improves vascular endothelial function by maintaining mitochondrial homeostasis under high glucose conditions

  • Sci Rep. 2023 Oct 2;13(1):16550. doi: 10.1038/s41598-023-43786-4.
Wahid Shah # 1,  Qiyue Zhao # 1,  Sen Wang 1,  Miaomiao Zhang 1,  Hongyu Ma 1,  Yue Guan 1,  Yi Zhang 1 2,  Yan Liu 3,  Chunhua Zhu 4,  Sheng Wang 1 2 5,  Xiangjian Zhang 2,  Jinghui Dong 6,  Huijie Ma 7 8 9 10
Affiliations

Affiliations

  • 1 Department of Physiology, Hebei Medical University, Shijiazhuang, 050017, Hebei, China.
  • 2 Hebei Collaborative Innovation Center for Cardio-Cerebrovascular Disease, Shijiazhuang, 050017, Hebei, China.
  • 3 Department of Endocrinology, The Third Hospital of Hebei Medical University, Ziqiang Road 139, Shijiazhuang, 050051, Hebei, China.
  • 4 Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, China.
  • 5 Key Laboratory of Neurophysiology of Hebei Province, Shijiazhuang, 050017, Hebei, China.
  • 6 Department of Physiology, Hebei Medical University, Shijiazhuang, 050017, Hebei, China. jinghuidong@126.com.
  • 7 Department of Physiology, Hebei Medical University, Shijiazhuang, 050017, Hebei, China. huijiema@hebmu.edu.cn.
  • 8 Hebei Collaborative Innovation Center for Cardio-Cerebrovascular Disease, Shijiazhuang, 050017, Hebei, China. huijiema@hebmu.edu.cn.
  • 9 Key Laboratory of Neurophysiology of Hebei Province, Shijiazhuang, 050017, Hebei, China. huijiema@hebmu.edu.cn.
  • 10 The Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Medical University, Shijiazhuang, 050017, Hebei, China. huijiema@hebmu.edu.cn.
  • # Contributed equally.
PMID: 37783713 DOI: 10.1038/s41598-023-43786-4
Abstract

Previous studies have shown that polydatin (Poly) confer cardioprotective effects. However, its underlying mechanisms remain elusive. This study showed that Poly (10 µM) treatment reversed the high glucose (HG)-induced decrease in acetylcholine-elicited vasodilation in aortas. Poly also improved the acetylcholine-induced vasodilation of aortic vessels isolated from diabetic rats. Meanwhile, Poly ameliorated the morphological damage of the thoracic aorta and improved the viability of HUVECs under HG conditions. Furthermore, analysis of the vasoprotective effect of Poly under HG conditions by transmission electron microscopy, Western blotting, and qPCR revealed that Poly improved endothelial Pyroptosis through the NLRP3/Caspase/1-IL-1β pathway, enhanced dynamin-related protein 1-mediated mitochondrial fission, and increased the mitochondrial membrane potential under HG conditions. In conclusion, Poly restored acetylcholine-induced vasodilation impaired by HG incubation, which was associated with reduced oxidation, inflammation, and Pyroptosis, the recovery of the mitochondrial membrane potential and maintenance of mitochondrial dynamic homeostasis of endothelial cells in the aortas.

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