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  2. Promoting oral absorption of Panax notoginseng saponins via thiolated trimethyl chitosan and wheat germ agglutinin-modified nanoformulation

Promoting oral absorption of Panax notoginseng saponins via thiolated trimethyl chitosan and wheat germ agglutinin-modified nanoformulation

  • Drug Deliv Transl Res. 2023 Oct 3. doi: 10.1007/s13346-023-01417-5.
Ruiyue Fang 1 Ying Zhao 1 Shiyuan Lin 1 Yue Wei 1 Hui Chen 2 3
Affiliations

Affiliations

  • 1 College of Pharmacy, Guilin Medical University, No. 1 Zhiyuan Road, Guilin, 541199, People's Republic of China.
  • 2 College of Pharmacy, Guilin Medical University, No. 1 Zhiyuan Road, Guilin, 541199, People's Republic of China. chenhui_gl@163.com.
  • 3 Pharmacology Laboratory of Prevention and Treatment of High Incidence of Disease, Guilin Medical University, Guilin, 541104, People's Republic of China. chenhui_gl@163.com.
Abstract

This study aimed to enhance the oral absorption of Panax notoginseng saponins (PNS) via nanoparticles modified with thiolated trimethyl chitosan (TMC-Cys) and wheat germ agglutinin (WGA), termed PP-WT NPs. In vitro investigations revealed that PP-WT NPs exhibited delayed release of PNS and a strong tolerance to the gastric acids and digestive Enzymes. Moreover, PP-WT NPs exhibited efficient cellular uptake and transport capabilities in the Caco-2/HT29-co-cultured cell model. In vivo animal experiments demonstrated that PP-WT NPs effectively overcame the mucus layer barrier, with the effective permeability coefficients of R1, Rg1, and Rb1 in the small intestine being 1.68, 1.64, and 1.63 times higher than those of free PNS, respectively. Taken together, thiolated trimethyl chitosan and wheat germ agglutinin-modified nanoparticles hold significant potential for improving the oral absorption of PNS, representing an attractive strategy for enhanced therapeutic efficacy.

Keywords

Caco-2&HT29-co-cultured cells; Nanoparticles; Oral absorption-promoting; Panax notoginseng saponins; Thiolated trimethyl chitosan; Wheat germ agglutinin.

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