2. Academic Validation
  3. SGCE promotes breast cancer stemness by promoting the transcription of FGF-BP1 by Sp1

SGCE promotes breast cancer stemness by promoting the transcription of FGF-BP1 by Sp1

  • J Biol Chem. 2023 Oct 12:105351. doi: 10.1016/j.jbc.2023.105351.
Ting Qiu 1,  Lei Hou 2,  Lina Zhao 3,  Xinye Wang 4,  Zhongmei Zhou 4,  Chuanyu Yang 4,  Huifeng Zhang 5,  Dewei Jiang 6,  Baowei Jiao 7,  Ceshi Chen 8
Affiliations

Affiliations

  • 1 Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences, KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases, Kunming Institute of Zoology, Kunming, Yunnan, China; Kunming College of Life sciences, University of Chinese Academy Sciences, Kunming, Yunnan, China.
  • 2 Department of Breast Disease, Henan Breast Cancer Center, Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou, 450008, China.
  • 3 Kunming College of Life sciences, University of Chinese Academy Sciences, Kunming, Yunnan, China; State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China.
  • 4 Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences, KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases, Kunming Institute of Zoology, Kunming, Yunnan, China.
  • 5 Department of Clinical Pharmacy, the First People's Hospital of Yunnan Province/the Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, China.
  • 6 Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences, KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases, Kunming Institute of Zoology, Kunming, Yunnan, China; Kunming College of Life sciences, University of Chinese Academy Sciences, Kunming, Yunnan, China. Electronic address: jiangdewei@mail.kiz.ac.cn.
  • 7 Department of Breast Disease, Henan Breast Cancer Center, Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou, 450008, China. Electronic address: jiaobaowei@mail.kiz.ac.cn.
  • 8 Academy of Biomedical Engineering, Kunming Medical University, Kunming 650500, China; The Third Affiliated Hospital, Kunming Medical University, Kunming 650118, China; Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences, KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases, Kunming Institute of Zoology, Kunming, Yunnan, China. Electronic address: chenc@mail.kiz.ac.cn.
PMID: 37838174 DOI: 10.1016/j.jbc.2023.105351
Abstract

Breast Cancer Stem Cells (BCSCs) are mainly responsible for poor prognosis, especially in triple-negative breast Cancer (TNBC). In a previous study, we demonstrated that ε-Sarcoglycan (SGCE), a type Ⅰ single-transmembrane protein, is a potential oncogene that promotes TNBC stemness by stabilizing EGFR. Here, we further found that SGCE depletion reduces BCSCs, partially through inhibiting the transcription of FGF-BP1, a secreted oncoprotein. Mechanistically, we demonstrate that SGCE could interact with the Sp1 transcription factor and translocate into the nucleus, which leads to an increase in the transcription of FGF-BP1 and the secreted FBF-BP1 activates FGF-FGFR signaling to promote Cancer cell stemness. The novel SGCE-Sp1-FGF-BP1 axis provides novel potential candidate diagnostic markers and therapeutic targets for TNBC.

Keywords

Breast cancer stem cells; TNBC; gene transcription; nuclear localization.

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