1. Academic Validation
  2. Catalpol ameliorates LPS-induced inflammatory response by activating AMPK/mTOR signaling pathway in rat intestinal epithelial cells

Catalpol ameliorates LPS-induced inflammatory response by activating AMPK/mTOR signaling pathway in rat intestinal epithelial cells

  • Eur J Pharmacol. 2023 Oct 26:960:176125. doi: 10.1016/j.ejphar.2023.176125.
Feng Gao 1 Qifu He 1 Shenghui Wu 1 Kang Zhang 1 Zhiming Xu 1 Jian Kang 1 Fusheng Quan 2
Affiliations

Affiliations

  • 1 Key Laboratory of Animal Biotechnology of the Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, China.
  • 2 Key Laboratory of Animal Biotechnology of the Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, China. Electronic address: quanfusheng@nwsuaf.edu.cn.
Abstract

Intestinal inflammation is a common clinical intestinal disease. Catalpol, a natural iridoid compound, has been shown to have anti-inflammatory, anti-oxidant and anti-apoptotic functions, but the mechanism of its protection against intestinal inflammation is still unclear. This study investigated the protective effect and potential mechanism of catalpol on the lipopolysaccharide (LPS)-induced inflammatory response of intestinal epithelial cell-6 (IEC-6). The results showed that catalpol could inhibit LPS-induced inflammatory response by dose-dependently reducing the release of inflammatory factors, such as tumor necrosis (TNF)-α, interleukin (IL)-1β and IL-6, and inhibiting the nuclear factor kappa-B (NF-κB) signaling pathway. Catalpol ameliorated cellular oxidative stress by reducing Reactive Oxygen Species (ROS) and malondialdehyde (MDA) levels and increasing superoxide dismutase (SOD) and Glutathione Peroxidase (GSH-PX) expression. Meanwhile, catalpol also inhibited cell Apoptosis, decreased the expression of B-cell lymphoma 2 (Bcl-2) - associated X (Bax), Caspase 3 and Caspase 9, and increased the expression of Bcl-2. This study found that catalpol activates AMP-activated protein kinase (AMPK) signaling pathway and inhibit mammalian target of rapamycin (mTOR) phosphorylationthe. In a further study, after inhibiting AMPK with dorsomorphin, the anti-inflammatory effects of catalpol were significantly reduced. Therefore, catalpol ameliorates LPS-induced inflammatory response by activating AMPK/mTOR signaling pathway in IEC-6 cells.

Keywords

AMPK/mTOR signaling pathway; Apoptosis; Catalpol; Intestinal inflammation; Oxidative stress.

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