2. Academic Validation
  3. Vitamin D3 can effectively and rapidly clear largemouth bass ranavirus by immunoregulation

Vitamin D3 can effectively and rapidly clear largemouth bass ranavirus by immunoregulation

  • Fish Shellfish Immunol. 2023 Nov 8:109213. doi: 10.1016/j.fsi.2023.109213.
Pengxu Wang 1,  Xingchen Huo 2,  Fengxia Zhao 2,  Qian Liu 2,  Qingqing Tian 2,  Chunrong Yang 3,  Jianguo Su 4
Affiliations

Affiliations

  • 1 Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, 266237, China; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China.
  • 2 Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China.
  • 3 College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
  • 4 Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, 266237, China; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: sujianguo@mail.hzau.edu.cn.
PMID: 37949380 DOI: 10.1016/j.fsi.2023.109213
Abstract

Largemouth bass ranavirus (LMBV) is a highly destructive pathogen that causes significant mortality rates among largemouth bass populations. Unfortunately, there is a dearth of drug development efforts specifically aimed at treating LMBV. To address this, our study sought to investigate the potential effectiveness of incorporating varying doses of VD3 into the diet as a treatment for LMBV. Through qRT-PCR and semi-qPCR, we observed significant suppression and clearance of LMBV pathogens in largemouth bass fed with 15000 IU/Kg and 20000 IU/Kg of VD3 within 14 days. In addition, VD3 treatment significantly increased the expression levels of key immune-related genes such as IL-1β, IFN-γ, Mx, and IgM. Encouragingly, we observed that VD3 significantly increased antioxidant and immune activities such as TSOD, TAOC and C3 in serum and maintained total protein levels. Additionally, tissue pathology sections highlighted a dose-dependent relationship between VD3 supplementation and tissue damage, with the 15000 IU and 20000 IU groups exhibiting minimal damage. In conclusion, a reasonable concentration of VD3 effectively reduced LMBV replication and tissue damages, while improved immune-related genes expression and serum biochemical indices. These findings declare the considerable therapeutic potential of VD3 supplementation for combating LMBV disease and provide an alternative treatment option for fish farming.

Keywords

Immunoprotection; Largemouth bass (Micropterus salmoides); Largemouth bass ranavirus (LMBV); Virus clearance; Vitamin D3 (VD3).

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