1. Academic Validation
  2. OPHTHALMIC WILD OLIVE (ACEBUCHE) OIL NANOEMULSIONS EXERT OCULOPROTECTIVE EFFECTS AGAINST OXIDATIVE STRESS INDUCED BY ARTERIAL HYPERTENSION

OPHTHALMIC WILD OLIVE (ACEBUCHE) OIL NANOEMULSIONS EXERT OCULOPROTECTIVE EFFECTS AGAINST OXIDATIVE STRESS INDUCED BY ARTERIAL HYPERTENSION

  • Int J Pharm. 2023 Nov 13:123602. doi: 10.1016/j.ijpharm.2023.123602.
A Santana-Garrido 1 M Durán-Lobato 2 A Mate 3 L Martín-Banderas 4 C M Vázquez 1
Affiliations

Affiliations

  • 1 Departamento de Fisiología, Facultad de Farmacia, Universidad de Sevilla. CL Profesor García González 2, 41012 Sevilla, Spain; Epidemiología Clínica y Riesgo Cardiovascular, Instituto de Biomedicina de Sevilla (IBIS), Hospital Universitario Virgen del Rocío - Consejo Superior de Investigaciones Científicas - Universidad de Sevilla. Avda. Manuel Siurot s/n, 41013 Sevilla, Spain.
  • 2 Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Sevilla. CL Profesor García González 2, 41012 Sevilla, Spain.
  • 3 Departamento de Fisiología, Facultad de Farmacia, Universidad de Sevilla. CL Profesor García González 2, 41012 Sevilla, Spain; Epidemiología Clínica y Riesgo Cardiovascular, Instituto de Biomedicina de Sevilla (IBIS), Hospital Universitario Virgen del Rocío - Consejo Superior de Investigaciones Científicas - Universidad de Sevilla. Avda. Manuel Siurot s/n, 41013 Sevilla, Spain. Electronic address: mate@us.es.
  • 4 Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Sevilla. CL Profesor García González 2, 41012 Sevilla, Spain. Electronic address: luciamartin@us.es.
Abstract

Oxidative stress plays a key role in several systemic and ocular diseases, including hypertensive eye diseases. In this context, we previously showed that oral administration of wild olive (acebuche, ACE) oil from Olea europaea var. sylvestris can counteract ocular damage secondary to arterial hypertension by modulating excess Reactive Oxygen Species (ROS) produced by the Enzyme nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. Therefore, this work describes the development of an ACE oil-based formulation for ocular administration as a local therapy to counteract hypertension-related oxidative damage. Specifically, ACE oil nanoemulsions (NEs) were successfully produced and characterized, exhibiting appropriate features for ophthalmic administration, including a nanometer size (<200 nm), moderate negative ZP, adequate osmolality and pH, and colloidal stability in biorelevant fluids. Likewise, the NEs presented a shear thinning behavior, especially convenient for ocular instillation. In vivo evaluation was performed through either intravitreal injection or topical ophthalmic administration in mice with hypertension induced via administration of Nω-nitro-L-arginine-methyl-ester (L-NAME). Both routes of administration reduced hypertensive morphological alterations and demonstrated a noticeable antioxidant effect thanks to the reduction of the activity/expression of NADPH Oxidase in cornea and retina. Thus, an ACE oil ophthalmic formulation represent a promising therapy for ocular pathologies associated with arterial hypertension.

Keywords

Acebuche oil; Cornea; L-NAME hypertension; Nanoemulsion; Oxidative stress; Retina.

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