2. Academic Validation
  3. Butyrate Alleviates High-Fat-Induced Metabolic Disorders Partially through Increasing Systematic Glutamine

Butyrate Alleviates High-Fat-Induced Metabolic Disorders Partially through Increasing Systematic Glutamine

  • J Agric Food Chem. 2023 Dec 18. doi: 10.1021/acs.jafc.3c08926.
Zeyu Fan 1 2,  Shaonan Wang 1 2,  Yingying Meng 1 2,  Chenglong Wen 1 2,  Meixue Xu 1 2,  Xiao Li 1 2
Affiliations

Affiliations

  • 1 College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China.
  • 2 Key Laboratory of Livestock Biology, Northwest A&F University, Yangling 712100, Shaanxi, China.
PMID: 38109504 DOI: 10.1021/acs.jafc.3c08926
Abstract

Obesity has emerged as a worldwide epidemic. Both butyrate and glutamine counteract obesity-related metabolic disorders; however, whether and how they synergistically cooperate with each Other remains a mystery. In the study, a high-fat diet (HFD, 60% calories from fat) was used to develop a model of obesity-related metabolic disorder and compared with administrated saline and sodium butyrate (SB, 300 mg/kg body weight) daily by gavage. Compared with HFD counterparts, oral administration of SB in mice exhibited significantly reduced body weight and fat mass and decreased hepatic triglyceride content. The targeted mass spectrum revealed that SB restored serum contents of glutamine, which were significantly decreased by HFD. Furthermore, SB significantly elevated the expression of glutamine synthetase (GS, encoded by GLUL) in the liver, accompanied by more enrichment of H3K27ac modifications within its promoter. In summary, the study verified the contribution of elevated glutamine to the beneficial effects of butyrate on metabolic disorders induced by a high-fat diet, providing a novel pathway for understanding how butyrate benefits metabolic homeostasis.

Keywords

H3K27ac; adipose tissue; butyrate; glutamine; high-fat diet.

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