1. Academic Validation
  2. JHD205, A Novel Abemaciclib Derivative, Exerts Antitumor Effects on Breast Cancer by CDK4/6

JHD205, A Novel Abemaciclib Derivative, Exerts Antitumor Effects on Breast Cancer by CDK4/6

  • Anticancer Agents Med Chem. 2024;24(6):400-411. doi: 10.2174/0118715206265751231204190204.
Jing Ji 1 Jingting Qin 1 Xiaoshuo Wang 1 Mingxiao Lv 1 Xiao Hou 1 Aixin Jing 1 Jiaojiao Zhou 1 Lingyi Zuo 1 Wenwen Liu 1 Jing Feng 1 Qilan Qian 1 Yuanyuan Liu 1 Xiujun Wang 1 Bin Liu 1
Affiliations

Affiliation

  • 1 Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, College of Pharmacy, Jiangsu Ocean University, Lianyungang, China.
Abstract

Background: Efficient targeted molecular therapeutics are needed for the treatment of triple-negative breast Cancer (TNBC), a highly invasive and difficult-to-treat form of breast Cancer associated with a poor prognosis.

Objectives: This study aims to evaluate the potential of selective CDK4/6 inhibitors as a therapeutic option for TNBC by impairing the cell cycle G1 phase through the inhibition of retinoblastoma protein (Rb) phosphorylation.

Methods: In this study, we synthesized a compound called JHD205, derived from the chemical structure of Abemaciclib, and examined its inhibitory effects on the malignant characteristics of TNBC cells.

Results: Our results demonstrated that JHD205 exhibited superior tumor growth inhibition compared to Abemaciclib in breast Cancer xenograft chicken embryo models. Western blot analysis revealed that JHD205 could dosedependently degrade CDK4 and CDK6 while also causing abnormal changes in Other proteins associated with CDK4/6, such as p-Rb, Rb, and E2F1. Moreover, JHD205 induced Apoptosis and DNA damage and inhibited DNA repair by upregulating Caspase3 and p-H2AX protein levels.

Conclusion: Collectively, our findings suggest that JHD205 holds promise as a potential treatment for breast carcinoma.

Keywords

Breast cancer; CDK4/6; DNA damage; antitumor activity.; cell phenotype; molecular docking.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-162985
    CDK4/6 Inhibitor
    CDK