2. Academic Validation
  3. Dual phosphorylation of DGK5-mediated PA burst regulates ROS in plant immunity

Dual phosphorylation of DGK5-mediated PA burst regulates ROS in plant immunity

  • Cell. 2024 Jan 11:S0092-8674(23)01404-6. doi: 10.1016/j.cell.2023.12.030.
Liang Kong 1,  Xiyu Ma 2,  Chao Zhang 1,  Sung-Il Kim 1,  Bo Li 3,  Yingpeng Xie 1,  In-Cheol Yeo 1,  Hem Thapa 3,  Sixue Chen 4,  Timothy P Devarenne 3,  Teun Munnik 5,  Ping He 6,  Libo Shan 7
Affiliations

Affiliations

  • 1 Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA.
  • 2 Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA. Electronic address: xiyu.ma@childrens.harvard.edu.
  • 3 Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA.
  • 4 Department of Biology, University of Mississippi, Oxford, MS 38677, USA.
  • 5 Department of Plant Cell Biology, Green Life Sciences Cluster, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam 1098XH, the Netherlands.
  • 6 Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA. Electronic address: pinghemi@umich.edu.
  • 7 Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biochemistry & Biophysics, Texas A&M University, College Station, TX 77843, USA. Electronic address: liboshan@umich.edu.
PMID: 38244548 DOI: 10.1016/j.cell.2023.12.030
Abstract

Phosphatidic acid (PA) and Reactive Oxygen Species (ROS) are crucial cellular messengers mediating diverse signaling processes in metazoans and Plants. How PA homeostasis is tightly regulated and intertwined with ROS signaling upon immune elicitation remains elusive. We report here that Arabidopsis diacylglycerol kinase 5 (DGK5) regulates plant pattern-triggered immunity (PTI) and effector-triggered immunity (ETI). The pattern recognition receptor (PRR)-associated kinase BIK1 phosphorylates DGK5 at Ser-506, leading to a rapid PA burst and activation of plant immunity, whereas PRR-activated intracellular MPK4 phosphorylates DGK5 at Thr-446, which subsequently suppresses DGK5 activity and PA production, resulting in attenuated plant immunity. PA binds and stabilizes the NADPH Oxidase RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD), regulating ROS production in plant PTI and ETI, and their potentiation. Our data indicate that distinct phosphorylation of DGK5 by PRR-activated BIK1 and MPK4 balances the homeostasis of cellular PA burst that regulates ROS generation in coordinating two branches of plant immunity.

Keywords

effector-triggered immunity; pattern-triggered immunity; phosphatidic acid; phosphorylation; reactive oxygen species.

Figures
Products