2. Academic Validation
  3. Improved CaP Nanoparticles for Nucleic Acid and Protein Delivery to Neural Primary Cultures and Stem Cells

Improved CaP Nanoparticles for Nucleic Acid and Protein Delivery to Neural Primary Cultures and Stem Cells

  • ACS Nano. 2024 Feb 13;18(6):4822-4839. doi: 10.1021/acsnano.3c09608.
Yu-Wen Chao 1 2,  Yen-Lurk Lee 3 4,  Ching-San Tseng 5,  Lily Ueh-Hsi Wang 3,  Kuo-Chiang Hsia 3,  Huatao Chen 6 7,  Jean-Michel Fustin 8,  Sayma Azeem 4 9,  Tzu-Tung Chang 4,  Chiung-Ya Chen 3,  Fan-Che Kung 4,  Yi-Ping Hsueh 3,  Yi-Shuian Huang 4 9 10,  Hsu-Wen Chao 1 2 11
Affiliations

Affiliations

  • 1 Department of Physiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110301, Taiwan.
  • 2 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110301, Taiwan.
  • 3 Institute of Molecular Biology, Academia Sinica, Taipei 115201, Taiwan.
  • 4 Institute of Biomedical Sciences, Academia Sinica, Taipei 115201, Taiwan.
  • 5 Department of Anatomy, School of Medicine, China Medical University, Taichung 40402, Taiwan.
  • 6 Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
  • 7 Key Laboratory of Animal Biotechnology of the Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, Shaanxi 712100, China.
  • 8 The University of Manchester, Faculty of Biology, Medicine and Health, Oxford Road, Manchester M13 9PL, U.K.
  • 9 Taiwan International Graduate Program in Interdisciplinary Neuroscience, National Yang-Ming Chao-Tung University and Academia Sinica, Taipei 115201, Taiwan.
  • 10 Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei 10002, Taiwan.
  • 11 Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
PMID: 38285698 DOI: 10.1021/acsnano.3c09608
Abstract

Efficiently delivering exogenous Materials into primary neurons and neural stem cells (NSCs) has long been a challenge in neurobiology. Existing methods have struggled with complex protocols, unreliable reproducibility, high immunogenicity, and cytotoxicity, causing a huge conundrum and hindering in-depth analyses. Here, we establish a cutting-edge method for transfecting primary neurons and NSCs, named teleofection, by a two-step process to enhance the formation of biocompatible calcium phosphate (CaP) nanoparticles. Teleofection enables both nucleic acid and protein transfection into primary neurons and NSCs, eliminating the need for specialized skills and equipment. It can easily fine-tune transfection efficiency by adjusting the incubation time and nanoparticle quantity, catering to various experimental requirements. Teleofection's versatility allows for the delivery of different cargos into the same Cell Culture, whether simultaneously or sequentially. This flexibility proves invaluable for long-term studies, enabling the monitoring of neural development and synapse plasticity. Moreover, teleofection ensures the consistent and robust expression of delivered genes, facilitating molecular and biochemical investigations. Teleofection represents a significant advancement in neurobiology, which has promise to transcend the limitations of current gene delivery methods. It offers a user-friendly, cost-effective, and reproducible approach for researchers, potentially revolutionizing our understanding of brain function and development.

Keywords

calcium phosphate; gene delivery; nanoparticles; neural stem cells; primary neurons; transfection.

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