1. Academic Validation
  2. Edwardsiella piscicida causes iron storage disorders by an autophagy pathway in fish monocytes/macrophages

Edwardsiella piscicida causes iron storage disorders by an autophagy pathway in fish monocytes/macrophages

  • Fish Shellfish Immunol. 2024 Mar:146:109417. doi: 10.1016/j.fsi.2024.109417.
Jingqi Ren 1 Xiaoyu Ma 1 Hengyi Hu 1 Dan Wang 1 Hao Sun 1 Jiaxi Liu 1 Xinyan Wang 1 Hong Zhou 2
Affiliations

Affiliations

  • 1 School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, People's Republic of China.
  • 2 School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, People's Republic of China. Electronic address: zhouhongzh@uestc.edu.cn.
Abstract

Edwardsiella piscicida (E. piscicida) is a gram-negative pathogen that survives in intracellular environment. Currently, the interplay between E. piscicida and host cells has not been completely explored. In this study, we found that E. piscicida disturbed iron homeostasis in grass carp monocytes/macrophages to maintain its own growth. Further investigation revealed the bacteria induced an increase of intracellular iron, which was subjected to the degradation of ferritin. Moreover, the Autophagy Inhibitor impeded the degradation of ferritin and increase of intracellular iron in E. piscicida-infected monocytes/macrophages, implying possible involvement of Autophagy response in the process of E. piscicida-broken iron homeostasis. Along this line, confocal microscopy observed that E. piscicida elicited the colocalization of ferritin with LC3-positive autophagosome in the monocytes/macrophages, indicating that E. piscicida mediated the degradation of ferritin possibly through the autophagic pathway. These results deepened our understanding of the interaction between E. piscicida and fish cells, hinting that the disruption of iron homeostasis was an important factor for pathogenicity of E. piscicida. They also indicated that Autophagy was a possible mechanism governing intracellular iron metabolism in response to E. piscicida Infection and might offer a new avenue for anti-E. piscicida strategies in the future.

Keywords

Autophagy; Edwardsiella piscicida; Ferritin; Grass carp; Iron homeostasis.

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