2. Academic Validation
  3. Application and Mechanism of Cryptolepine and Neocryptolepine Derivatives as T3SS Inhibitors for Control of Bacterial Leaf Blight on Rice

Application and Mechanism of Cryptolepine and Neocryptolepine Derivatives as T3SS Inhibitors for Control of Bacterial Leaf Blight on Rice

  • J Agric Food Chem. 2024 Apr 3;72(13):6988-6997. doi: 10.1021/acs.jafc.4c00214.
Jiang Shao 1,  Zhi-Jun Zhang 2,  Yu Shi 1,  Wei-Qi Jiang 2,  Faisal Siddique 1,  Liangye Chen 1,  Genyan Liu 3,  Jiakai Zhu 2,  Xiong-Fei Luo 2,  Ying-Qian Liu 2,  Jun-Xia An 2,  Cheng-Jie Yang 2,  Zi-Ning Cui 1
Affiliations

Affiliations

  • 1 National Key Laboratory of Green Pesticide, Integrative Microbiology Research Centre, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.
  • 2 School of Pharmacy, Lanzhou University, Lanzhou 730000, China.
  • 3 Hubei Key Laboratory of Novel Reactor and Green Chemical Technology, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430205, China.
PMID: 38506764 DOI: 10.1021/acs.jafc.4c00214
Abstract

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv oryzae (Xoo) is extremely harmful to rice production. The traditional control approach is to use bactericides that target key Bacterial growth factors, but the selection pressure on the pathogen makes resistant strains the dominant Bacterial strains, leading to a decline in bactericidal efficacy. Type III secretion system (T3SS) is a conserved and critical virulence factor in most Gram-negative bacteria, and its expression or absence does not affect Bacterial growth, rendering it an ideal target for creating drugs against Gram-negative pathogens. In this work, we synthesized a range of derivatives from cryptolepine and neocryptolepine. We found that compound Z-8 could inhibit the expression of Xoo T3SS-related genes without affecting the growth of bacteria. an in vivo bioassay showed that compound Z-8 could effectively reduce the hypersensitive response (HR) induced by Xoo in tobacco and reduce the pathogenicity of Xoo in rice. Furthermore, it exhibited synergy in control of Bacterial leaf blight when combined with the quorum quenching Bacterial F20.

Keywords

T3SS; Xanthomonas oryzae pv oryzae; bacterial leaf blight; cryptolepine and neocryptolepine derivatives; inhibitor; synthesis.

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