2. Academic Validation
  3. PLGA-based nanocarriers for combined delivery of colchicine and purpurin 18 in cancer therapy: Multimodal approach employing cancer cell spheroids

PLGA-based nanocarriers for combined delivery of colchicine and purpurin 18 in cancer therapy: Multimodal approach employing cancer cell spheroids

  • Int J Pharm. 2024 Apr 26:657:124170. doi: 10.1016/j.ijpharm.2024.124170.
Alma Lucia Villela Zumaya 1,  Vladimíra Svobodová Pavlíčková 2,  Silvie Rimpelová 3,  Markéta Štějdířová 1,  Michal Fulem 1,  Ivana Křížová 4,  Pavel Ulbrich 2,  Pavel Řezanka 1,  Fatima Hassouna 5
Affiliations

Affiliations

  • 1 Faculty of Chemical Engineering, University of Chemistry and Technology Prague, 166 28 Prague 6, Czech Republic.
  • 2 Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, 166 28 Prague 6, Czech Republic.
  • 3 Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, 166 28 Prague 6, Czech Republic. Electronic address: silvie.rimpelova@vscht.cz.
  • 4 Faculty of Biotechnology, University of Chemistry and Technology Prague, 166 28 Prague 6, Czech Republic.
  • 5 Faculty of Chemical Engineering, University of Chemistry and Technology Prague, 166 28 Prague 6, Czech Republic. Electronic address: fatima.hassouna@vscht.cz.
PMID: 38679244 DOI: 10.1016/j.ijpharm.2024.124170
Abstract

Improving the Anticancer efficacy of chemotherapeutic drugs and photosensitizers requires innovative multifunctional nanoplatforms. This study introduces a chemo- and phototherapeutic Drug Delivery system (DDS) based on poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs), both PEGylated and non-PEGylated, with a mean size of 200 ± 75 nm. Colchicine (Colch) and purpurin18 (P18) were co-encapsulated into these NPs, and their in vitro drug release profiles were investigated. The Anticancer potential of these systems was evaluated across various cell lines (i.e., CaCo-2, PC-3, MCF-7, and MRC-5 cells), demonstrating enhanced NP uptake by Cancer cells compared to free drugs. Co-administration of Colch and P18 in 2D and 3D cell line models exhibited a synergistic effect, harnessing both chemotherapeutic and photodynamic effects, leading to higher Cancer cell elimination efficacy. This newly developed multifunctional DDS presents a promising platform for combined chemo- and photodynamic therapy in Cancer treatment.

Keywords

Cancer cell spheroids; Colchicine; Drug delivery system; Multifunctional nanoplatform; PEGylated PLGA nanoparticles; PLGA nanoparticles; Purpurin 18.

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