2. Academic Validation
  3. All-in-one hydrogel patches with sprayed bFGF-loaded GelMA microspheres for infected wound healing studies

All-in-one hydrogel patches with sprayed bFGF-loaded GelMA microspheres for infected wound healing studies

  • Int J Pharm. 2024 Jun 10:658:124205. doi: 10.1016/j.ijpharm.2024.124205.
Wenzhang Jin 1,  Shuqi Shen 2,  Xiaoniuyue Xu 3,  Xueting Xie 2,  Xingjian Zhou 2,  Xiang Su 4,  Lina Wu 2,  Shunfu Wang 2,  Lijiang Zhang 2,  Bicheng Chen 5,  Fajing Yang 6
Affiliations

Affiliations

  • 1 Department of Vascular Surgery, The First Affiliated Hospital of Wenzhou Medical University, Zhejiang Province Wenzhou 325000, PR China; Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, PR China; Department of Colorectal Surgery, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310000, PR China.
  • 2 Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, PR China.
  • 3 Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, PR China; Department of Hand Surgery, The First Affiliated Hospital of Wenzhou Medical University, Zhejiang Province Wenzhou 325000, PR China.
  • 4 Department of Vascular Surgery, The First Affiliated Hospital of Wenzhou Medical University, Zhejiang Province Wenzhou 325000, PR China; Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, PR China.
  • 5 Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, PR China. Electronic address: chenbicheng@hotmail.com.
  • 6 Department of Vascular Surgery, The First Affiliated Hospital of Wenzhou Medical University, Zhejiang Province Wenzhou 325000, PR China; Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, PR China. Electronic address: yangfj25@126.com.
PMID: 38734278 DOI: 10.1016/j.ijpharm.2024.124205
Abstract

The current wound healing process faces numerous challenges such as Bacterial infection, inflammation and oxidative stress. However, wound dressings used to promote wound healing, are not well suited to meet the clinical needs. Hyaluronic acid (HA) not only has excellent water absorption and good biocompatibility but facilitates cell function and tissue regeneration. Dopamine, on the Other hand, increases the overall viscosity of the hydrogel and possesses antioxidant property. Furthermore, chitosan exhibits outstanding performance in antimicrobial, anti-inflammatory and antioxidant activities. Basic Fibroblast Growth Factor (bFGF) is conducive to cell proliferation and migration, vascular regeneration and wound healing. Hence, we designed an all-in-one hydrogel patch containing dopamine and chitosan framed by hyaluronic acid (HDC) with sprayed gelatin methacryloyl (GelMA) microspheres loaded with bFGF (HDC-bFGF). The hydrogel patch exhibits excellent adhesive, anti-inflammatory, antioxidant and Antibacterial properties. In vitro experiments, the HDC-bFGF hydrogel patch not only showed significant inhibitory effect on RAW cell inflammation and Staphylococcus aureus (S. aureus) growth but also effectively scavenged free radicals, in addition to promoting the migration of 3 T3 cells. In the mice acute infected wound model, the HDC-bFGF hydrogel patch adhered to the wound surface greatly accelerated the healing process via its anti-inflammatory and antioxidant activities, Bacterial inhibition and pro-vascularization effects. Therefore, the multifunctional HDC-bFGF hydrogel patch holds great promise for clinical application.

Keywords

Hyaluronic acid; Hydrogel patch; Infected wound; Microsphere; bFGF.

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